αCP1 mediates stabilization of hTERT mRNA by autocrine human growth hormone

B. Starling Emerald, Yong Chen, Tao Zhu, Zhe Zhu, Kok Onn Lee, Peter D. Gluckman, Peter E. Lobie

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

We herein demonstrate that autocrine human growth hormone production in human mammary carcinoma cells results in increased telomerase activity as a result of specific up-regulation of telomerase catalytic subunit (human telomerase reverse transcriptase (hTERT)) mRNA and protein. This increase in hTERT gene expression is not due to increased transcriptional activation of the hTERT promoter but is the result of increased stability of hTERT mRNA exerted by CU-rich cis-regulatory sequences present in the 3′-untranslated region of TERT mRNA. Autocrine human growth hormone up-regulates two poly(C)-binding proteins, αCP1 and αCP2, which bind to these cis-regulatory elements and stabilize hTERT mRNA. We have therefore demonstrated that post-transcriptional modulation of the level of hTERT mRNA is one mechanism for regulation of cellular telomerase activity.

Original languageEnglish
Pages (from-to)680-690
Number of pages11
JournalJournal of Biological Chemistry
Volume282
Issue number1
DOIs
Publication statusPublished - Jan 5 2007
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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