TY - JOUR
T1 - αCP1 mediates stabilization of hTERT mRNA by autocrine human growth hormone
AU - Emerald, B. Starling
AU - Chen, Yong
AU - Zhu, Tao
AU - Zhu, Zhe
AU - Lee, Kok Onn
AU - Gluckman, Peter D.
AU - Lobie, Peter E.
PY - 2007/1/5
Y1 - 2007/1/5
N2 - We herein demonstrate that autocrine human growth hormone production in human mammary carcinoma cells results in increased telomerase activity as a result of specific up-regulation of telomerase catalytic subunit (human telomerase reverse transcriptase (hTERT)) mRNA and protein. This increase in hTERT gene expression is not due to increased transcriptional activation of the hTERT promoter but is the result of increased stability of hTERT mRNA exerted by CU-rich cis-regulatory sequences present in the 3′-untranslated region of TERT mRNA. Autocrine human growth hormone up-regulates two poly(C)-binding proteins, αCP1 and αCP2, which bind to these cis-regulatory elements and stabilize hTERT mRNA. We have therefore demonstrated that post-transcriptional modulation of the level of hTERT mRNA is one mechanism for regulation of cellular telomerase activity.
AB - We herein demonstrate that autocrine human growth hormone production in human mammary carcinoma cells results in increased telomerase activity as a result of specific up-regulation of telomerase catalytic subunit (human telomerase reverse transcriptase (hTERT)) mRNA and protein. This increase in hTERT gene expression is not due to increased transcriptional activation of the hTERT promoter but is the result of increased stability of hTERT mRNA exerted by CU-rich cis-regulatory sequences present in the 3′-untranslated region of TERT mRNA. Autocrine human growth hormone up-regulates two poly(C)-binding proteins, αCP1 and αCP2, which bind to these cis-regulatory elements and stabilize hTERT mRNA. We have therefore demonstrated that post-transcriptional modulation of the level of hTERT mRNA is one mechanism for regulation of cellular telomerase activity.
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U2 - 10.1074/jbc.M600224200
DO - 10.1074/jbc.M600224200
M3 - Article
C2 - 17085453
AN - SCOPUS:33846988278
SN - 0021-9258
VL - 282
SP - 680
EP - 690
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 1
ER -