TY - JOUR
T1 - 3D culture of mouse gastric stem cells using porous microcarriers
AU - AlKhatib, Razan
AU - Al Naqbi, Ali Abdullah Hilal
AU - Naciri, Mariam
AU - Al-Majmaie, Rasoul
AU - Saseedharan, Prashanth
AU - Karam, Sherif M.
AU - Al-Rubeai, Mohamed
N1 - Funding Information:
This work was supported by funding from the UAEU start-up fund (31N154-2013) and UAEU fund #31R073.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - The lining epithelium of the stomach includes multipotent stem cells which undergo proliferation and migration-associated differentiation. These cells give rise to multiple cell lineages that produce mucus, acid, pepsinogen and various hormones/peptides. A 3D culture for stem cells would facilitate identification of the factors that control proliferation and/or differentiation programs. Here, we report on the use of disk-like ImmobaSil HD silicone-rubber matrix based microcarriers that are permeable to oxygen and reduce the creation of toxic environment within the center of the microcarrierd for culturing the mouse gastric stem (mGS) cells. We define several parameters that affect the initial cell attachment such as size of cell inoculum, serum concentration, mode and speed of agitation. We show that although such a microcarrier allows for attachment and growth of gastric stem cells, it does not lend itself and does not support the functional differentiation of such cells.
AB - The lining epithelium of the stomach includes multipotent stem cells which undergo proliferation and migration-associated differentiation. These cells give rise to multiple cell lineages that produce mucus, acid, pepsinogen and various hormones/peptides. A 3D culture for stem cells would facilitate identification of the factors that control proliferation and/or differentiation programs. Here, we report on the use of disk-like ImmobaSil HD silicone-rubber matrix based microcarriers that are permeable to oxygen and reduce the creation of toxic environment within the center of the microcarrierd for culturing the mouse gastric stem (mGS) cells. We define several parameters that affect the initial cell attachment such as size of cell inoculum, serum concentration, mode and speed of agitation. We show that although such a microcarrier allows for attachment and growth of gastric stem cells, it does not lend itself and does not support the functional differentiation of such cells.
KW - 3D culture
KW - Cell culture
KW - Gastric epithelium
KW - Microcarriers
KW - Stem cells
UR - http://www.scopus.com/inward/record.url?scp=85016137799&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85016137799&partnerID=8YFLogxK
U2 - 10.2741/S481
DO - 10.2741/S481
M3 - Article
C2 - 27814583
AN - SCOPUS:85016137799
SN - 1945-0516
VL - 9
SP - 172
EP - 179
JO - Frontiers in Bioscience - Scholar
JF - Frontiers in Bioscience - Scholar
IS - 1
ER -