A closed chamber method for performing biochemical experiments at accurate concentrations of volatile agents

Murat Oz, Alp Alptekin, Meral Dinc

Research output: Contribution to journalArticlepeer-review

Abstract

Experiments with volatile agents such as general anesthetics present difficulties in maintaining defined concentrations of these agents during in-vitro experimental conditions. In conventional filtration apparatuses, due to their partition between liquid and vapor phases (to open air or headspaces of the incubation vehicles), some degree of inaccuracy in calculated concentrations of these agents may occur in experiments using these types of chambers. In the present study, a method is described which permits the performance of biochemical experiments in a closed system in which the concentrations of a volatile agent, desflurane, in the liquid phase of the assay environment can be maintained constant for a relatively long time period.

Original languageEnglish
Pages (from-to)95-100
Number of pages6
JournalJournal of Biochemical and Biophysical Methods
Volume55
Issue number1
DOIs
Publication statusPublished - Jan 31 2003
Externally publishedYes

Keywords

  • Biochemical methods
  • Desflurane
  • Headspace
  • Volatile agents

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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