Abstract
Experiments with volatile agents such as general anesthetics present difficulties in maintaining defined concentrations of these agents during in-vitro experimental conditions. In conventional filtration apparatuses, due to their partition between liquid and vapor phases (to open air or headspaces of the incubation vehicles), some degree of inaccuracy in calculated concentrations of these agents may occur in experiments using these types of chambers. In the present study, a method is described which permits the performance of biochemical experiments in a closed system in which the concentrations of a volatile agent, desflurane, in the liquid phase of the assay environment can be maintained constant for a relatively long time period.
Original language | English |
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Pages (from-to) | 95-100 |
Number of pages | 6 |
Journal | Journal of Biochemical and Biophysical Methods |
Volume | 55 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 31 2003 |
Externally published | Yes |
Keywords
- Biochemical methods
- Desflurane
- Headspace
- Volatile agents
ASJC Scopus subject areas
- Biophysics
- Biochemistry