A comparative analysis of anti-lipidemic potential of soybean (Glycine max) protein hydrolysates obtained from different ripening stages: Identification, and molecular interaction mechanisms of novel bioactive peptides

Amna Alnuaimi, Feyisola Fisayo Ajayi, Marwa Hamdi, Priti Mudgil, Hina Kamal, Chee Yuen Gan, Sajid Maqsood

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

This study aims to investigate the potentials of mature (MSPHs) and young (YSPHs) soybean enzymatic protein hydrolysates for the inhibition of pancreatic lipase (PL) and cholesterol esterase (C-Ease) enzymes. Higher proteins degradation levels were recorded with Bromelain compared to Flavourzyme and Alcalase, and upon hydrolysis, improved PL and C-Ease inhibition performances were displayed compared to unhydrolyzed proteins. Afterwards, six PHs with potent anti-lipidemic activities were selected for sequencing using LC-MS QTOF and molecular binding studies. Peptides FPFPRPPHQ, QCCAFEM, FAPEFLK from MSPHs and SFFFPFELPRE, FMYL, PFLL, FPLL, LPHF from YSPHs were predicted to possess potent inhibitory activities against PL. Furthermore, FPFPRPPHQ, FMYL, MMLM from MSPHs, and SFFFPFELPRE from YSPHs were predicted to be potent inhibitors of C-Ease. FPFPRPPHQ and SFFFPFELPRE derived from MSPHs and YSPHs, respectively, demonstrated effective inhibition potentialities against both PL and C-Ease. Therefore, mature and young soybean-derived protein hydrolysates could be recognized as a potential ingredient in the management of hypercholesterolemia.

Original languageEnglish
Article number134192
JournalFood Chemistry
Volume402
DOIs
Publication statusPublished - Feb 15 2023

Keywords

  • Bioactive peptides
  • Cholesterol esterase
  • Hydrolysates
  • Hypercholesterolemia
  • Pancreatic lipase
  • Soybean protein

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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