A family of acyclic brevinin-1 peptides from the skin of the Ryukyu brown frog Rana okinavana

J. Michael Conlon, Agnes Sonnevend, Thierry Jouenne, Laurent Coquet, David Cosquer, Hubert Vaudry, Shawichi Iwamuro

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)


The 24 amino-acid residue antimicrobial peptide, brevinin-1 is synthesized in the skins of a wide range of species of Eurasian and North American frogs belonging to the genus Rana. All previously characterized brevinin-1 peptides contain the cyclic heptapeptide domain Cys 18-(Xaa) 4-Lys-Cys 24 at the COOH-terminus of the molecule. Four structurally related peptides were isolated from an extract of the skin of the Ryukyu brown frog Rana okinavana. The amino acid sequences of the peptides [Phe-(Xaa) 4-Ile-(Xaa) 2-Leu-Ala-Lys-Gly-Leu-Pro-Ser-Leu- Ile-Xaa-Leu-Xaa-Lys-Lys·NH 2] identified them as members of the brevinin-1 family that lacked the COOH-terminal cyclic domain but contained a C-terminally α-amidated residue. It is suggested, as one possibility, that the Cys 18 in the brevinin-1 consensus sequence has been deleted and the Cys 24 residue has mutated to a glycine that acts as substrate for peptidyl-glycine α-amidating monooxygenase. The peptides potently inhibited the growth of Escherichia coli and Staphylococcus aureus confirming that a cyclic domain is not necessary for antimicrobial activity. A fifth peptide (SFLNFFKGAA 10KNLLAAGLDK 20LKCKISGTQC 30), that also displayed broad-spectrum antimicrobial activity, was isolated from the skin extract and showed structural similarity with members of the ranatuerin-2 family previously isolated from the skin of North American ranid frogs.

Original languageEnglish
Pages (from-to)185-190
Number of pages6
Issue number2
Publication statusPublished - Feb 2005
Externally publishedYes


  • Antimicrobial
  • Brevinin-1
  • Frog skin
  • Ranatuerin-2

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience


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