A family of antimicrobial peptides related to japonicin-2 isolated from the skin of the chaochiao brown frog Rana chaochiaoensis

J. Michael Conlon; Jérôme Leprince; Hubert Vaudry; Hu Jiansheng; Per F. Nielsen

doi:10.1016/j.cbpc.2006.07.007

A family of antimicrobial peptides related to japonicin-2 isolated from the skin of the chaochiao brown frog Rana chaochiaoensis

J. Michael Conlon, Jérôme Leprince, Hubert Vaudry, Hu Jiansheng, Per F. Nielsen

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Four structurally-related peptides with antimicrobial activity were isolated from an extract of the skin of the Chinese brown frog, Rana chaochiaoensis Liu, 1946. Determination of their primary structures revealed that they are members of the japonicin-2 family, previously identified only in the skin of the Japanese brown frog, R. japonica. Japonicin-2CHa (FVLPLLGILPKELCIVLKKNC) represented the most abundant peptide in the extract but its growth-inhibitory potency against Staphylococcus aureus (minimum inhibitory concentration, MIC = 100 μM) and Escherichia coli (MIC > 100 μM) was appreciably less than that of the more cationic japonicin-2 (FGLPMLSILPKALCILLKRKC). The high degree of structural similarity of japonicin-2CHb (VVPAFVLLKKAICIMLKRNC) with japonicin-2CHc (K⁹ → R), and japonicin-2CHd (L¹⁶ → F) is suggestive of recent gene duplication events. The data indicate a close phylogenetic relationship between R. chaochiaoensis and R. japonica but demonstrate that the species are not conspecific.

Original language	English
Pages (from-to)	101-105
Number of pages	5
Journal	Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume	144
Issue number	1
DOIs	https://doi.org/10.1016/j.cbpc.2006.07.007
Publication status	Published - Sept 2006
Externally published	Yes

Keywords

Antimicrobial peptide
Frog skin
HPLC purification
Japonicin-2

ASJC Scopus subject areas

Biochemistry
Physiology
Aquatic Science
Animal Science and Zoology
Toxicology
Cell Biology
Health, Toxicology and Mutagenesis

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10.1016/j.cbpc.2006.07.007

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A family of antimicrobial peptides related to japonicin-2 isolated from the skin of the chaochiao brown frog Rana chaochiaoensis. / Conlon, J. Michael; Leprince, Jérôme; Vaudry, Hubert et al.
In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 144, No. 1, 09.2006, p. 101-105.

Research output: Contribution to journal › Article › peer-review

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abstract = "Four structurally-related peptides with antimicrobial activity were isolated from an extract of the skin of the Chinese brown frog, Rana chaochiaoensis Liu, 1946. Determination of their primary structures revealed that they are members of the japonicin-2 family, previously identified only in the skin of the Japanese brown frog, R. japonica. Japonicin-2CHa (FVLPLLGILPKELCIVLKKNC) represented the most abundant peptide in the extract but its growth-inhibitory potency against Staphylococcus aureus (minimum inhibitory concentration, MIC = 100 μM) and Escherichia coli (MIC > 100 μM) was appreciably less than that of the more cationic japonicin-2 (FGLPMLSILPKALCILLKRKC). The high degree of structural similarity of japonicin-2CHb (VVPAFVLLKKAICIMLKRNC) with japonicin-2CHc (K9 → R), and japonicin-2CHd (L16 → F) is suggestive of recent gene duplication events. The data indicate a close phylogenetic relationship between R. chaochiaoensis and R. japonica but demonstrate that the species are not conspecific.",

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AU - Jiansheng, Hu

AU - Nielsen, Per F.

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A family of antimicrobial peptides related to japonicin-2 isolated from the skin of the chaochiao brown frog Rana chaochiaoensis

Abstract

Keywords

ASJC Scopus subject areas

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