TY - JOUR
T1 - A family of antimicrobial peptides related to japonicin-2 isolated from the skin of the chaochiao brown frog Rana chaochiaoensis
AU - Conlon, J. Michael
AU - Leprince, Jérôme
AU - Vaudry, Hubert
AU - Jiansheng, Hu
AU - Nielsen, Per F.
N1 - Funding Information:
This work was supported by an Interdisciplinary Grant (03/12-8-03-01), an Individual Research Grant (01-03-8-11/06), and a Faculty Support Grant (NP/06/02) from the United Arab Emirates University.
PY - 2006/9
Y1 - 2006/9
N2 - Four structurally-related peptides with antimicrobial activity were isolated from an extract of the skin of the Chinese brown frog, Rana chaochiaoensis Liu, 1946. Determination of their primary structures revealed that they are members of the japonicin-2 family, previously identified only in the skin of the Japanese brown frog, R. japonica. Japonicin-2CHa (FVLPLLGILPKELCIVLKKNC) represented the most abundant peptide in the extract but its growth-inhibitory potency against Staphylococcus aureus (minimum inhibitory concentration, MIC = 100 μM) and Escherichia coli (MIC > 100 μM) was appreciably less than that of the more cationic japonicin-2 (FGLPMLSILPKALCILLKRKC). The high degree of structural similarity of japonicin-2CHb (VVPAFVLLKKAICIMLKRNC) with japonicin-2CHc (K9 → R), and japonicin-2CHd (L16 → F) is suggestive of recent gene duplication events. The data indicate a close phylogenetic relationship between R. chaochiaoensis and R. japonica but demonstrate that the species are not conspecific.
AB - Four structurally-related peptides with antimicrobial activity were isolated from an extract of the skin of the Chinese brown frog, Rana chaochiaoensis Liu, 1946. Determination of their primary structures revealed that they are members of the japonicin-2 family, previously identified only in the skin of the Japanese brown frog, R. japonica. Japonicin-2CHa (FVLPLLGILPKELCIVLKKNC) represented the most abundant peptide in the extract but its growth-inhibitory potency against Staphylococcus aureus (minimum inhibitory concentration, MIC = 100 μM) and Escherichia coli (MIC > 100 μM) was appreciably less than that of the more cationic japonicin-2 (FGLPMLSILPKALCILLKRKC). The high degree of structural similarity of japonicin-2CHb (VVPAFVLLKKAICIMLKRNC) with japonicin-2CHc (K9 → R), and japonicin-2CHd (L16 → F) is suggestive of recent gene duplication events. The data indicate a close phylogenetic relationship between R. chaochiaoensis and R. japonica but demonstrate that the species are not conspecific.
KW - Antimicrobial peptide
KW - Frog skin
KW - HPLC purification
KW - Japonicin-2
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U2 - 10.1016/j.cbpc.2006.07.007
DO - 10.1016/j.cbpc.2006.07.007
M3 - Article
C2 - 16928470
AN - SCOPUS:33748079108
SN - 1532-0456
VL - 144
SP - 101
EP - 105
JO - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
IS - 1
ER -