A fluorogenic assay for methylglyoxal

Fozia Shaheen, Anatoly Shmygol, Naila Rabbani, Paul J. Thornalley

Research output: Contribution to journalReview articlepeer-review

21 Citations (Scopus)


MG (methylglyoxal) is a potent glycating agent and an endogenous reactive dicarbonyl metabolite formed in all live cells and organisms. It is an important precursor of AGEs (advanced glycation end-products) and is implicated in aging and disease. MG is assayed by derivatization by 1,2-diaminobenzene derivatives in cell extracts. Such assays are not applicable to high sample throughput, subcellular, live-cell and in vivo estimations. The use of fluorogenic probes designed for NO (nitric oxide) detection in biological samples and living cells has inadvertently provided probes for the detection of dicarbonyls such as MG. We describe the application of DAF-2 (4,5-diaminofluorescein) and DAR-1 (4,5-diaminorhodamine) for the detection of MG in cell-free systems and application for high-throughput assay of glyoxalase activity and assay of glucose degradation products in peritoneal dialysis fluids. DAF-2 and DAR-1, as for related BODIPY probes, do not have sufficient sensitivity to detect MG in live cells. Care will also be required to control for NO and dehydroascorbate co-detection and interference from peroxidase catalysing the degradation of probes to MG and glyoxal. Fluorogenic detection of MG, however, has great potential to facilitate the assay of MG and to advance towards that capability of imaging this product in live cells in vitro and small animals in vivo.

Original languageEnglish
Pages (from-to)548-555
Number of pages8
JournalBiochemical Society Transactions
Issue number2
Publication statusPublished - Apr 2014
Externally publishedYes


  • 4,5-diaminofluorescein
  • 4,5-diaminorhodamine
  • Glucose degradation product
  • Glyoxalase
  • Methylglyoxal
  • Nitric oxide

ASJC Scopus subject areas

  • Biochemistry


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