A high-quality genome assembly and annotation of Thielaviopsis punctulata DSM102798

Gouthaman P. Purayil; Esam Eldin Saeed; Archana M. Mathai; Khaled A. El-Tarabily; Synan F. AbuQamar

doi:10.1038/s41597-024-03458-y

A high-quality genome assembly and annotation of Thielaviopsis punctulata DSM102798

Gouthaman P. Purayil, Esam Eldin Saeed, Archana M. Mathai, Khaled A. El-Tarabily, Synan F. AbuQamar

Department of Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Black scorch disease (BSD), caused by the fungal pathogen Thielaviopsis punctulata (Tp) DSM102798, poses a significant threat to date palm cultivation in the United Arab Emirates (UAE). In this study, Chicago and Hi-C libraries were prepared as input for the Dovetail HiRise pipeline to scaffold the genome of Tp DSM102798. We generated an assembly with a total length of 28.23 Mb comprising 1,256 scaffolds, and the assembly had a contig N50 of 18.56 kb, L50 of three, and a BUSCO completeness score of 98.6% for 758 orthologous genes. Annotation of this assembly produced 7,169 genes and 3,501 Gene Ontology (GO) terms. Compared to five other Thielaviopsis genomes, Tp DSM102798 exhibited the highest continuity with a cumulative size of 27.598 Mb for the first seven scaffolds, surpassing the assemblies of all examined strains. These findings offer a foundation for targeted strategies that enhance date palm resistance against BSD, and foster more sustainable and resilient agricultural systems.

Original language	English
Article number	745
Journal	Scientific data
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41597-024-03458-y
Publication status	Published - Dec 2024

ASJC Scopus subject areas

Statistics and Probability
Information Systems
Education
Computer Science Applications
Statistics, Probability and Uncertainty
Library and Information Sciences

Access to Document

10.1038/s41597-024-03458-y

Cite this

@article{0c4bfa905d324724b8040cf12f3a71ed,

title = "A high-quality genome assembly and annotation of Thielaviopsis punctulata DSM102798",

abstract = "Black scorch disease (BSD), caused by the fungal pathogen Thielaviopsis punctulata (Tp) DSM102798, poses a significant threat to date palm cultivation in the United Arab Emirates (UAE). In this study, Chicago and Hi-C libraries were prepared as input for the Dovetail HiRise pipeline to scaffold the genome of Tp DSM102798. We generated an assembly with a total length of 28.23 Mb comprising 1,256 scaffolds, and the assembly had a contig N50 of 18.56 kb, L50 of three, and a BUSCO completeness score of 98.6% for 758 orthologous genes. Annotation of this assembly produced 7,169 genes and 3,501 Gene Ontology (GO) terms. Compared to five other Thielaviopsis genomes, Tp DSM102798 exhibited the highest continuity with a cumulative size of 27.598 Mb for the first seven scaffolds, surpassing the assemblies of all examined strains. These findings offer a foundation for targeted strategies that enhance date palm resistance against BSD, and foster more sustainable and resilient agricultural systems.",

author = "Purayil, {Gouthaman P.} and Saeed, {Esam Eldin} and Mathai, {Archana M.} and El-Tarabily, {Khaled A.} and AbuQamar, {Synan F.}",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2024.",

year = "2024",

month = dec,

doi = "10.1038/s41597-024-03458-y",

language = "English",

volume = "11",

journal = "Scientific data",

issn = "2052-4463",

publisher = "Nature Research",

number = "1",

}

TY - JOUR

T1 - A high-quality genome assembly and annotation of Thielaviopsis punctulata DSM102798

AU - Purayil, Gouthaman P.

AU - Saeed, Esam Eldin

AU - Mathai, Archana M.

AU - El-Tarabily, Khaled A.

AU - AbuQamar, Synan F.

N1 - Publisher Copyright: © The Author(s) 2024.

PY - 2024/12

Y1 - 2024/12

N2 - Black scorch disease (BSD), caused by the fungal pathogen Thielaviopsis punctulata (Tp) DSM102798, poses a significant threat to date palm cultivation in the United Arab Emirates (UAE). In this study, Chicago and Hi-C libraries were prepared as input for the Dovetail HiRise pipeline to scaffold the genome of Tp DSM102798. We generated an assembly with a total length of 28.23 Mb comprising 1,256 scaffolds, and the assembly had a contig N50 of 18.56 kb, L50 of three, and a BUSCO completeness score of 98.6% for 758 orthologous genes. Annotation of this assembly produced 7,169 genes and 3,501 Gene Ontology (GO) terms. Compared to five other Thielaviopsis genomes, Tp DSM102798 exhibited the highest continuity with a cumulative size of 27.598 Mb for the first seven scaffolds, surpassing the assemblies of all examined strains. These findings offer a foundation for targeted strategies that enhance date palm resistance against BSD, and foster more sustainable and resilient agricultural systems.

AB - Black scorch disease (BSD), caused by the fungal pathogen Thielaviopsis punctulata (Tp) DSM102798, poses a significant threat to date palm cultivation in the United Arab Emirates (UAE). In this study, Chicago and Hi-C libraries were prepared as input for the Dovetail HiRise pipeline to scaffold the genome of Tp DSM102798. We generated an assembly with a total length of 28.23 Mb comprising 1,256 scaffolds, and the assembly had a contig N50 of 18.56 kb, L50 of three, and a BUSCO completeness score of 98.6% for 758 orthologous genes. Annotation of this assembly produced 7,169 genes and 3,501 Gene Ontology (GO) terms. Compared to five other Thielaviopsis genomes, Tp DSM102798 exhibited the highest continuity with a cumulative size of 27.598 Mb for the first seven scaffolds, surpassing the assemblies of all examined strains. These findings offer a foundation for targeted strategies that enhance date palm resistance against BSD, and foster more sustainable and resilient agricultural systems.

UR - http://www.scopus.com/inward/record.url?scp=85198074576&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85198074576&partnerID=8YFLogxK

U2 - 10.1038/s41597-024-03458-y

DO - 10.1038/s41597-024-03458-y

M3 - Article

C2 - 38982096

AN - SCOPUS:85198074576

SN - 2052-4463

VL - 11

JO - Scientific data

JF - Scientific data

IS - 1

M1 - 745

ER -

A high-quality genome assembly and annotation of Thielaviopsis punctulata DSM102798

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this