A high-resolution comparative radiation hybrid map of equine chromosome 4q12-q22

C. Dierks; S. Mömke; C. Drögemüller; T. Leeb; B. P. Chowdhary; O. Distl

doi:10.1111/j.1365-2052.2006.01510.x

A high-resolution comparative radiation hybrid map of equine chromosome 4q12-q22

C. Dierks, S. Mömke, C. Drögemüller, T. Leeb, B. P. Chowdhary, O. Distl

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

In this study, we present a comprehensive 5000-rad radiation hybrid map of a 40-cM region on equine chromosome 4 (ECA4) that contains quantitative trait loci for equine osteochondrosis. We mapped 29 gene-associated sequence tagged site markers using primers designed from equine expressed sequence tags or BAC clones in the ECA4q12-q22 region. Three blocks of conserved synteny, showing two chromosomal breakpoints, were identified in the segment of ECA4q12-q22. Markers from other segments of HSA7q mapped to ECA13p and ECA4p, and a region of HSA7p was homologous to ECA13p. Therefore, we have improved the resolution of the human-equine comparative map, which allows the identification of candidate genes underlying traits of interest.

Original language	English
Pages (from-to)	513-517
Number of pages	5
Journal	Animal Genetics
Volume	37
Issue number	5
DOIs	https://doi.org/10.1111/j.1365-2052.2006.01510.x
Publication status	Published - Oct 2006
Externally published	Yes

Keywords

Comparative map
Equine chromosome 4
Equus caballus
Fluorescent in situ hybridization
Radiation hybrid mapping

ASJC Scopus subject areas

Animal Science and Zoology
Genetics

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10.1111/j.1365-2052.2006.01510.x

Cite this

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title = "A high-resolution comparative radiation hybrid map of equine chromosome 4q12-q22",

abstract = "In this study, we present a comprehensive 5000-rad radiation hybrid map of a 40-cM region on equine chromosome 4 (ECA4) that contains quantitative trait loci for equine osteochondrosis. We mapped 29 gene-associated sequence tagged site markers using primers designed from equine expressed sequence tags or BAC clones in the ECA4q12-q22 region. Three blocks of conserved synteny, showing two chromosomal breakpoints, were identified in the segment of ECA4q12-q22. Markers from other segments of HSA7q mapped to ECA13p and ECA4p, and a region of HSA7p was homologous to ECA13p. Therefore, we have improved the resolution of the human-equine comparative map, which allows the identification of candidate genes underlying traits of interest.",

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AU - Dierks, C.

AU - Mömke, S.

AU - Drögemüller, C.

AU - Leeb, T.

AU - Chowdhary, B. P.

AU - Distl, O.

PY - 2006/10

Y1 - 2006/10

N2 - In this study, we present a comprehensive 5000-rad radiation hybrid map of a 40-cM region on equine chromosome 4 (ECA4) that contains quantitative trait loci for equine osteochondrosis. We mapped 29 gene-associated sequence tagged site markers using primers designed from equine expressed sequence tags or BAC clones in the ECA4q12-q22 region. Three blocks of conserved synteny, showing two chromosomal breakpoints, were identified in the segment of ECA4q12-q22. Markers from other segments of HSA7q mapped to ECA13p and ECA4p, and a region of HSA7p was homologous to ECA13p. Therefore, we have improved the resolution of the human-equine comparative map, which allows the identification of candidate genes underlying traits of interest.

AB - In this study, we present a comprehensive 5000-rad radiation hybrid map of a 40-cM region on equine chromosome 4 (ECA4) that contains quantitative trait loci for equine osteochondrosis. We mapped 29 gene-associated sequence tagged site markers using primers designed from equine expressed sequence tags or BAC clones in the ECA4q12-q22 region. Three blocks of conserved synteny, showing two chromosomal breakpoints, were identified in the segment of ECA4q12-q22. Markers from other segments of HSA7q mapped to ECA13p and ECA4p, and a region of HSA7p was homologous to ECA13p. Therefore, we have improved the resolution of the human-equine comparative map, which allows the identification of candidate genes underlying traits of interest.

KW - Comparative map

KW - Equine chromosome 4

KW - Equus caballus

KW - Fluorescent in situ hybridization

KW - Radiation hybrid mapping

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A high-resolution comparative radiation hybrid map of equine chromosome 4q12-q22

Abstract

Keywords

ASJC Scopus subject areas

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