A multienzyme system immobilized on surface-modified metal–organic framework for enhanced CO2 hydrogenation

Shadeera Rouf, Yaser E. Greish, Bart Van der Bruggen, Sulaiman Al-Zuhair

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Hydrogenating carbon dioxide to formate using formate dehydrogenase (FDH) is a sustainable approach for CO2 mitigation. Herein, we developed a biocatalytic system with cofactor regeneration by immobilizing multiple enzymes, namely FDH, carbonic anhydrase (CA), and glutamate dehydrogenase (GDH), on a hydrophobic surface modified MOF, SA-HKUST-1. The adsorption kinetics of the multiple enzymes on the SA-HKUST-1 surface were described using pseudo second-order model, while the equilibrium followed Freundlich isotherm. Formate production by the enzymes immobilized on SA-HKUST-1 was 3.75 times higher than that achieved by free enzymes and 8.4 times higher than that of FDH immobilized alone on SA-HKUST-1. The hydrophobic interaction between the enzymes and the support altered the secondary structure of enzymes, and the immobilized enzymes retained 94% of their activity after four reuse cycles. This study provides novel insights into the combined effect of hydrophobic support and multiple enzymes on the catalytic efficiency and stability of FDH. These findings can provide a basis for developing a highly stable biocatalytic system with cofactor regeneration for continuous hydrogenation of CO2 to formate at the industrial level.

Original languageEnglish
Article number100234
JournalCarbon Resources Conversion
Issue number4
Publication statusPublished - Dec 2024


  • CO hydrogenation
  • Cofactor regeneration
  • Formate
  • HKUST-1
  • Hydrophobic metal–organic framework
  • Multienzyme system

ASJC Scopus subject areas

  • Catalysis
  • Materials Science (miscellaneous)
  • Fuel Technology
  • Process Chemistry and Technology


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