A novel multiplex assay for simultaneously analysing 13 rapidly mutating Y-STRs

Rashed Alghafri, Will Goodwin, Arwin Ralf, Manfred Kayser, Sibte Hadi

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)


Abstract A multiplex polymerase chain reaction (PCR) assay (RM-Yplex) was developed which is capable of simultaneously amplifying 13 recently introduced rapidly mutating Y-STR markers (RM Y-STRs). This multiplex assay is expected to aid human identity testing in forensic and other applications to improve differentiating unrelated males and allow separating related males. The 13 RM Y-STR markers included in the multiplex are: DYF387S1, DYF399S1, DYF403S1ab, DYF404S1, DYS449, DYS518, DYS526ab, DYS547, DYS570, DYS576, DYS612, DYS626 and DYS627. This study reflects the proof of concept to analyse all currently known RM Y-STRs simultaneously and describes the optimization of the multiplex assay. The RM-Yplex assay generated complete RM Y-STR profiles down to 62.5 pg of male template DNA, and from male-female DNA mixtures at all ratios tested. We herewith introduce and make available for widespread use in forensic and anthropological studies, an effective and sensitive single multiplex assay for simultaneous genotyping of 13 RM Y-STRs.

Original languageEnglish
Article number1339
Pages (from-to)91-98
Number of pages8
JournalForensic Science International: Genetics
Publication statusPublished - May 1 2015
Externally publishedYes


  • Haplotypes
  • Rapidly mutating Y-STRs
  • Y-STRs
  • Y-chromosomal STRs

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Genetics


Dive into the research topics of 'A novel multiplex assay for simultaneously analysing 13 rapidly mutating Y-STRs'. Together they form a unique fingerprint.

Cite this