TY - JOUR
T1 - A specific histamine-stimulated phosphoprotein in isolated parietal cells
AU - Modlin, Irvin M.
AU - Oddsdottir, Margret
AU - Adrian, Thomas E.
AU - Zdon, Michael J.
AU - Zucker, Karl A.
AU - Goldenring, James R.
N1 - Funding Information:
The authors thank Dr. Howard Rassmussen for invaluable guidance and criticisms of this work, and Dr. Robert DeLorenzo for the use of his Loats two-dimensional digitizing analyzer. This work was supported by a grant from the Veterans Administration to I.M.M.
PY - 1987/4
Y1 - 1987/4
N2 - Histamine-stimulated phosphorylation was studied in isolated rabbit parietal cells. Secretion of acid, as assessed by aminopyrine uptake, was linear at 15 min of stimulation with histamine. By utilizing two dimensional gels, a specific 30,000-Da protein (pp30) was identified whose phosphorylation was prominently stimulated by histamine after 15 min of incubation. The pp30 protein displayed an isoelectric point of 6.0. Furthermore, cAMP-dependent pp30 phosphorylation could also be demonstrated in vitro in a preparation of parietal cell cytosol. The results suggest that pp30 may represent an important histamine-stimulated cAMP-dependent phosphoprotein involved in the initiation or maintenance of parietal cell secretion.
AB - Histamine-stimulated phosphorylation was studied in isolated rabbit parietal cells. Secretion of acid, as assessed by aminopyrine uptake, was linear at 15 min of stimulation with histamine. By utilizing two dimensional gels, a specific 30,000-Da protein (pp30) was identified whose phosphorylation was prominently stimulated by histamine after 15 min of incubation. The pp30 protein displayed an isoelectric point of 6.0. Furthermore, cAMP-dependent pp30 phosphorylation could also be demonstrated in vitro in a preparation of parietal cell cytosol. The results suggest that pp30 may represent an important histamine-stimulated cAMP-dependent phosphoprotein involved in the initiation or maintenance of parietal cell secretion.
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U2 - 10.1016/0022-4804(87)90168-5
DO - 10.1016/0022-4804(87)90168-5
M3 - Article
C2 - 3033399
AN - SCOPUS:0023255636
SN - 0022-4804
VL - 42
SP - 348
EP - 353
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 4
ER -