A specific histamine-stimulated phosphoprotein in isolated parietal cells

Irvin M. Modlin, Margret Oddsdottir, Thomas E. Adrian, Michael J. Zdon, Karl A. Zucker, James R. Goldenring

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Histamine-stimulated phosphorylation was studied in isolated rabbit parietal cells. Secretion of acid, as assessed by aminopyrine uptake, was linear at 15 min of stimulation with histamine. By utilizing two dimensional gels, a specific 30,000-Da protein (pp30) was identified whose phosphorylation was prominently stimulated by histamine after 15 min of incubation. The pp30 protein displayed an isoelectric point of 6.0. Furthermore, cAMP-dependent pp30 phosphorylation could also be demonstrated in vitro in a preparation of parietal cell cytosol. The results suggest that pp30 may represent an important histamine-stimulated cAMP-dependent phosphoprotein involved in the initiation or maintenance of parietal cell secretion.

Original languageEnglish
Pages (from-to)348-353
Number of pages6
JournalJournal of Surgical Research
Issue number4
Publication statusPublished - Apr 1987
Externally publishedYes

ASJC Scopus subject areas

  • Surgery


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