A spectroscopy based prototype for the noninvasive detection of diabetes from human saliva using nanohybrids acting as nanozyme

Lopamudra Roy, Susmita Mondal, Neha Bhattacharyya, Ria Ghosh, Amrita Banerjee, Soumendra Singh, Arpita Chattopadhyay, Saleh A. Ahmed, Rabab S. Jassas, Munirah M. Al-Rooqi, Ziad Moussa, Ismail I. Althagafi, Debasish Bhattacharya, Kallol Bhattacharya, Asim Kumar Mallick, Samir Kumar Pal

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The recent prediction of diabetes to be a global pandemic invites a detection strategy preferably non-invasive, and bloodless to manage the disease and the associated complications. Here, we have synthesized chitosan polymer functionalized, organic–inorganic bio-compatible nano-hybrids of Mn3O4 nanoparticles, and characterized it by utilizing several optical methodologies for the structural characterization which shows the Michaelis Menten (MM) kinetics for glucose and alpha-amylase protein (well-known diabetes biomarkers). We have also studied the potentiality for the detection of alpha-amylase in human salivary secretion which is reported to be strongly correlated with uncontrolled hyperglycemia. Finally, we have developed a prototype for the measurement of glucose (LOD of 0.38 mg/dL, LOQ of 1.15 mg/dL) and HbA1c (LOD of 0.15% and LOQ of 0.45%) utilizing the basic knowledge in the study for the detection of uncontrolled hyperglycemia at the point-of-care. With the limited number of clinical trials, we have explored the potential of our work in combating the diabetic pandemic across the globe in near future.

Original languageEnglish
Article number17306
JournalScientific reports
Volume13
Issue number1
DOIs
Publication statusPublished - Dec 2023

ASJC Scopus subject areas

  • General

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