A tool for measuring oxygen via phosphorescence

Sami Shaban, Harvey S. Penefsky, Abdul Kader Souid

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Accurate measurements of dissolved O2 as a function of time have numerous biological and chemical applications. Detection of O2 using a Pd phosphor complex is based on the principle that the phosphorescence of this probe is inversely related to dissolved [O2]. Biologic samples are flashed with red light using special instrumentation that captures emitted light and a customized software program that determines [O2] as function of time. O2 concentration is calculated by fitting the decay of the phosphorescence to an exponential curve. Twelve tasks were identified, which allow full control and customization of the data acquisition, storage and analysis. The program used Microsoft Visual Basic 6 (VB6), Microsoft Access Database 2007, and a Universal Library component that allowed direct reading from the PCI-DAS 4020/12 I/O Board. A relational database was designed to store experiments, pulses and pulse metadata, including phosphorescence decay rates. This combination of experimental setup and software program can be used on wide range of experiments. The tool has now been used to conduct over 2, 000 experiments during the last two years, as it permits reliable measurements of cellular O2 consumption over several hours.

Original languageEnglish
Title of host publicationWMSCI 2012 - The 16th World Multi-Conference on Systemics, Cybernetics and Informatics, Proceedings
Pages118-120
Number of pages3
Publication statusPublished - 2012
Event16th World Multi-Conference on Systemics, Cybernetics and Informatics, WMSCI 2012 - Oralndo, FL, United States
Duration: Jul 17 2012Jul 20 2012

Publication series

NameWMSCI 2012 - The 16th World Multi-Conference on Systemics, Cybernetics and Informatics, Proceedings
Volume2

Other

Other16th World Multi-Conference on Systemics, Cybernetics and Informatics, WMSCI 2012
Country/TerritoryUnited States
CityOralndo, FL
Period7/17/127/20/12

Keywords

  • Data acquisition
  • Oxygen
  • Peak detection
  • Phosphorescence
  • Slope calculation

ASJC Scopus subject areas

  • Artificial Intelligence
  • Computer Networks and Communications
  • Information Systems

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