@article{c18e182af98b4e9891600fdec2dc70bc,
title = "Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays",
abstract = "The yeast SWI/SNF complex is required for the transcription of several yeast genes and has been shown to alter nucleosome structure in an ATP-dependent reaction. In this study, we show that the complex stimulated in vitro transcription from nucleosome templates in an activation domain-dependent manner. Transcription stimulation by SWI/SNF required an activation domain with which it directly interacts. The acidic activation domains of VP16, Gcn4, Swi5, and Hap4 interacted directly with the purified SWI/SNF complex and with the SWI/SNF complex in whole-cell extracts. The similarity of activation domain interactions and transcriptional stimulation between SWI/SNF and the SAGA histone acetyltransferase complex may account for their apparent overlapping functions in vivo.",
author = "Neely, \{Kristen E.\} and Hassan, \{Ahmed H.\} and Wallberg, \{Annika E.\} and Steger, \{David J.\} and Cairns, \{Bradley R.\} and Wright, \{Anthony P.H.\} and Workman, \{Jerry L.\}",
note = "Funding Information: We thank D. Stillman, M. Green, J. Reese, S. Berger, B. F. Pugh, and K. Ikeda for providing expression plasmids, J. Reese for an αTaf30 antibody, T. Owen-Hughes, J. C{\^o}t{\'e}, and R. Utley for preparing Gal4–VP16 and Gal4–glutamine, and M. Vignali for assistance in preparing figures. We thank K. Natarajan and A. Hinnebusch for testing SWI fractions for mediator subunits and sharing unpublished information. We thank the members of the Workman, Simpson, and Reese labs at Penn State for many valuable discussions. This work was supported by a grant from NIGMS to J. L. W. A grant was awarded to A. P. H. W. from the Swedish Natural Sciences Research Council. A. E. W. was awarded fellowships by the Swedish Medical Research Council (K98-03RM-12413) and the Erik and Edith Fernstr{\"o}m Foundation. D. J. S. was a Postdoctoral Associate of HHMI, and J. L. W. is an HHMI Associate Investigator.",
year = "1999",
month = oct,
doi = "10.1016/S1097-2765(00)80216-6",
language = "English",
volume = "4",
pages = "649--655",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "4",
}