Adhesion of microorganisms to bovine submaxillary mucin coatings: Effect of coating deposition conditions

Ibraheem A. Bushnak, Fatima H. Labeed, Richard P. Sear, Joseph L. Keddie

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

The adhesion of Staphylococcus epidermidis, Escherichia coli, and Candida albicans on mucin coatings was evaluated to explore the feasibility of using the coating to increase the infection resistance of biomaterials. Coatings of bovine submaxillary mucin (BSM) were deposited on a base layer consisting of a poly(acrylic acid-b-methyl methacrylate) (PAA-b-PMMA) diblock copolymer. This bi-layer system exploits the mucoadhesive interactions of the PAA block to aid the adhesion of mucin to the substratum, whereas the PMMA block prevents dissolution of the coating in aqueous environments. The thickness of the mucin coating was adjusted by varying the pH of the solution from which it was deposited. Thin mucin coatings decreased the numbers of bacteria but increased the numbers of C. albicans adhering to the copolymer and control surfaces. Increasing the mucin film thickness resulted in a further lowering of the density of adhering S. epidermidis cells, but it did not affect the density of E. coli. In contrast, the density of C. albicans increased with an increase in mucin thickness.

Original languageEnglish
Pages (from-to)387-397
Number of pages11
JournalBiofouling
Volume26
Issue number4
DOIs
Publication statusPublished - May 2010
Externally publishedYes

Keywords

  • Bayesian statistics
  • Candida albicans
  • Coatings
  • Escherichia coli
  • Mucin
  • Mucoadhesion
  • Poly(acrylic acid)
  • Staphylococcus epidermidis

ASJC Scopus subject areas

  • Aquatic Science
  • Applied Microbiology and Biotechnology
  • Water Science and Technology

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