Adsorption study of albumin bound bilirubin over date pits activated carbon

Toxicity of protein bound toxins is increased due to liver failure, various methods have been developed for their removal and adsorption plays a key role in all these methods. Bilirubin is an albumin bound toxin and albumin act as a standard clinical marker for liver failure patients. Activated carbon is used widely to remove albuminbound bilirubin proteins. Activated carbon (AC) from date pits (DP) is reported for the first time in the study and the adsorption capacity of activated date pit carbon (ADCP) was measured from the residual concentration of bilirubin in the solution by taking readings of different time intervals using uv-visible spectroscopy. Changes in the surface morphology of date pit activated carbon before and after activation were examined by scanning electron microscopy which showed the pore size of activated carbon. Various amount of activated carbon was added indifferent concentrations. The adsorption of bilirubin at wavelength of 416 nm and 350 nm was examined and studied. The results showed that there was a decrease of the bilirubin in the presence of activated carbon whereas the control which contains albumin bound bilirubin in PBS remain stable. This study also showed that bilirubin is better adsorbed on higher amount of activated carbon derived from the precursor date pits.