TY - JOUR
T1 - Advanced Selection Methodologies for DNAzymes in Sensing and Healthcare Applications
AU - Kumar, Sandeep
AU - Jain, Shikha
AU - Dilbaghi, Neeraj
AU - Ahluwalia, Amrik Singh
AU - Hassan, Ashraf Aly
AU - Kim, Ki Hyun
N1 - Funding Information:
S.K. thanks the Department of Science and Technology (DST), the Government of India, the University of Nebraska (UNL) at Lincoln, the Daugherty Water for Food Institute (DWFI), and the Indo-US Science and Technology Forum (IUSSTF) for financial support through the Water Advanced Research and Innovation (WARI) Program (award IUSSTF/WARI/2018/F-029-2018, 03-01-2018) as well as the Department of Biotechnology (DBT), Government of India (award BT/PR18868/BCE/8/1370/2016, 31-01-2018), and DST-PURSE (Promotion of University Research and Scientific Excellence) via GJUS&T, Hisar, under PURSE program SR/PURSE Phase 2/40(G). S.J. thanks the University Grants Commission (UGC), India, for providing financial assistance in the form of a Junior Research Fellowship [award F.16-6 (Dec. 2016)/2017 (NET), 12-09-2017]. K.H.K. acknowledges grant support from the National Research Foundation of Korea (NRF) funded by the MSIP (Ministry of Science, ICT, and Future Planning), Government of Korea (grant 2016R1E1A1A01940995) as well as the support made by the R&D Center for Green Patrol Technologies through the R&D for Global Top Environmental Technologies funded by the Ministry of Environment (MOE 2018001850001).
Funding Information:
S.K. thanks the Department of Science and Technology (DST) , the Government of India , the University of Nebraska (UNL) at Lincoln , the Daugherty Water for Food Institute (DWFI) , and the Indo-US Science and Technology Forum (IUSSTF) for financial support through the Water Advanced Research and Innovation (WARI) Program (award IUSSTF/WARI/2018/F-029-2018 , 03-01-2018 ) as well as the Department of Biotechnology (DBT), Government of India (award BT/PR18868/BCE/8/1370/2016 , 31-01-2018 ), and DST-PURSE (Promotion of University Research and Scientific Excellence) via GJUS&T, Hisar, under PURSE program SR/PURSE Phase 2/40(G). S.J. thanks the University Grants Commission (UGC) , India, for providing financial assistance in the form of a Junior Research Fellowship [award F.16-6 (Dec. 2016)/2017 (NET), 12-09-2017 ]. K.H.K. acknowledges grant support from the National Research Foundation of Korea (NRF) funded by the MSIP (Ministry of Science, ICT, and Future Planning) , Government of Korea (grant 2016R1E1A1A01940995 ) as well as the support made by the R&D Center for Green Patrol Technologies through the R&D for Global Top Environmental Technologies funded by the Ministry of Environment (MOE 2018001850001).
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2019/3
Y1 - 2019/3
N2 - DNAzymes have been widely explored owing to their excellent catalytic activity in a broad range of applications, notably in sensing and biomedical devices. These newly discovered applications have built high hopes for designing novel catalytic DNAzymes. However, the selection of efficient DNAzymes is a challenging process but one that is of crucial importance. Initially, systemic evolution of ligands by exponential enrichment (SELEX) was a labor-intensive and time-consuming process, but recent advances have accelerated the automated generation of DNAzyme molecules. This review summarizes recent advances in SELEX that improve the affinity and specificity of DNAzymes. The thriving generation of new DNAzymes is expected to open the door to several healthcare applications. Therefore, a significant portion of this review is dedicated to various biological applications of DNAzymes, such as sensing, therapeutics, and nanodevices. In addition, discussion is further extended to the barriers encountered for the real-life application of these DNAzymes to provide a foundation for future research.
AB - DNAzymes have been widely explored owing to their excellent catalytic activity in a broad range of applications, notably in sensing and biomedical devices. These newly discovered applications have built high hopes for designing novel catalytic DNAzymes. However, the selection of efficient DNAzymes is a challenging process but one that is of crucial importance. Initially, systemic evolution of ligands by exponential enrichment (SELEX) was a labor-intensive and time-consuming process, but recent advances have accelerated the automated generation of DNAzyme molecules. This review summarizes recent advances in SELEX that improve the affinity and specificity of DNAzymes. The thriving generation of new DNAzymes is expected to open the door to several healthcare applications. Therefore, a significant portion of this review is dedicated to various biological applications of DNAzymes, such as sensing, therapeutics, and nanodevices. In addition, discussion is further extended to the barriers encountered for the real-life application of these DNAzymes to provide a foundation for future research.
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U2 - 10.1016/j.tibs.2018.11.001
DO - 10.1016/j.tibs.2018.11.001
M3 - Review article
C2 - 30559045
AN - SCOPUS:85058377173
SN - 0376-5067
VL - 44
SP - 190
EP - 213
JO - Trends in Biochemical Sciences
JF - Trends in Biochemical Sciences
IS - 3
ER -