TY - JOUR
T1 - Aescin and diosmin each alone or in low dose- combination ameliorate liver damage induced by carbon tetrachloride in rats
AU - El-Dakhly, Sara Mahmmoud
AU - Salama, Abeer Abdallah Ali
AU - Hassanin, Soha Osama Mahmoud
AU - Yassen, Noha Nazeeh
AU - Hamza, Alaaeldin Ahmed
AU - Amin, Amr
N1 - Funding Information:
Partially support by Al‑Jalila Foundation No. 21S098 was provided for Amr Amin.
Funding Information:
Authors thank Al‑Jalila Foundation No. 21S098 for partially funding this study for Amr Amin.
Publisher Copyright:
© 2020 The Author(s).
PY - 2020/5/27
Y1 - 2020/5/27
N2 - Objective: This study evaluated hepatoprotective effect of aescin (AES) and diosmin (DIO), individually or in low-dose combination in chemically induced liver injury in rats. Rats were divided into 6 groups; Group 1, control, Group 2, injected with a single dose of a mixture of corn oil and carbon tetrachloride (CCl4) to induce hepatic toxicity. Before CCl4 injection, Groups 3-6 were treated daily for 14 days with silymarin (SIL) (200 mg/kg), aescin (AES; 3.6 & 1.75 mg/kg), Diosmin (DIO; 100 & 50 mg/kg). Serum samples were analyzed for different liver function, oxidative stress and antioxidant markers. Moreover, inflammation and tissue damage were confirmed by histological staining of liver tissue sections. Results: Results indicated that CCl4 elevated serum levels of all assessed liver function markers and decreased levels of key antioxidants. Administration of AES and/or DIO significantly reversed all those CCl4-induced effects. Histopathological study showed disruption of the hepatic architecture, necrosis and inflammatory cells and depositions of glycogen and protein in the tissues of CCl4-treated group. Pretreatment with DIO and/or AES significantly improved histopathological structure of liver tissue. In conclusion, low-dose combination of AES and DIO exhibited significant and preferential hepatoprotective activity compared to individual treatment with AES or DIO.
AB - Objective: This study evaluated hepatoprotective effect of aescin (AES) and diosmin (DIO), individually or in low-dose combination in chemically induced liver injury in rats. Rats were divided into 6 groups; Group 1, control, Group 2, injected with a single dose of a mixture of corn oil and carbon tetrachloride (CCl4) to induce hepatic toxicity. Before CCl4 injection, Groups 3-6 were treated daily for 14 days with silymarin (SIL) (200 mg/kg), aescin (AES; 3.6 & 1.75 mg/kg), Diosmin (DIO; 100 & 50 mg/kg). Serum samples were analyzed for different liver function, oxidative stress and antioxidant markers. Moreover, inflammation and tissue damage were confirmed by histological staining of liver tissue sections. Results: Results indicated that CCl4 elevated serum levels of all assessed liver function markers and decreased levels of key antioxidants. Administration of AES and/or DIO significantly reversed all those CCl4-induced effects. Histopathological study showed disruption of the hepatic architecture, necrosis and inflammatory cells and depositions of glycogen and protein in the tissues of CCl4-treated group. Pretreatment with DIO and/or AES significantly improved histopathological structure of liver tissue. In conclusion, low-dose combination of AES and DIO exhibited significant and preferential hepatoprotective activity compared to individual treatment with AES or DIO.
KW - Aescin
KW - Carbon tetrachloride
KW - Diosmin
KW - Liver
KW - Rats
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U2 - 10.1186/s13104-020-05094-2
DO - 10.1186/s13104-020-05094-2
M3 - Article
C2 - 32460808
AN - SCOPUS:85085539804
SN - 1756-0500
VL - 13
JO - BMC Research Notes
JF - BMC Research Notes
IS - 1
M1 - 259
ER -