Aging impairs skeletal muscle mitochondrial bioenergetic function

Pedro Alexandre Figueiredo, Scott K. Powers, Rita M. Ferreira, Hans Joachim Appell, José A. Duarte

Research output: Contribution to journalArticlepeer-review

86 Citations (Scopus)


This study investigated the influence of age on the functional status of mitochondria isolated from skeletal muscle of C57BL/6 mice aged 3 and 18 months. We hypothesized that skeletal muscle mitochondria isolated from aged animals will exhibit a decreased respiratory function. Mitochondrial respiratory functional measures (ie, State 3 and 4 respiration, respiratory control ratio and number of nanomoles of ADP phosphorylated by nanomoles of O2 consumed per mitochondrion) and biochemical markers of oxidative damage (aconitase activity, protein carbonyl derivatives, sulfhydryl groups, and malondialdehyde) were measured in isolated mitochondrial suspensions. Along with traditional tests of mitochondrial function, an in vitro repetitive ADP-stimulation test was used to evaluate the mitochondrial capacity to reestablish the homeostatic balance between successive ADP stimulations. The number of mitochondria per mitochondrial suspension, calculated by transmission electron microscopy, was used to normalize functional and biochemical data. Our results confirm the existence of an age-associated decline in mitochondrial function of mixed skeletal muscle, which is significantly correlated with higher levels of mitochondrial oxidative damage.

Original languageEnglish
Pages (from-to)21-33
Number of pages13
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Issue number1
Publication statusPublished - Jan 2009
Externally publishedYes


  • Aging
  • Mitochondria
  • Respiratory function

ASJC Scopus subject areas

  • General Medicine


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