TY - JOUR
T1 - Alpha-interferon inhibits spontaneous and induced DNA synthesis in hairy cell leukemia
AU - Hassan, Inaam Bashir
AU - Gronowitz, J. Simon
AU - Carlsson, Mats
AU - Sundstrom, Christer
N1 - Funding Information:
Acknowledgements This study was supported by the Swedish Cancer Society (project No. 1937-B89-06XB and No. 1942-B91- 08XBC) and from the Swedish Commission for Technical Cooperation Program between the Sudanese Ministry of Health and Uppsala University. The technical assistance of Ms Anneli Kraft is gratefully acknowledged.
PY - 1992
Y1 - 1992
N2 - The effect of alpha-interferon (αIFN) on spontaneous or induced proliferation of isolated peripheral blood mononuclear cells from 5 hairy cell leukemia patients was studied. a-IFN inhibited the low spontaneous proliferation of B-1y7 positive hairy cells (HCs) and also the proliferation induced by tumour necrosis factor (TNF). Interleukin-2 did not affect HCs, but induced CD4 positive T cells to proliferate, an effect which αIFN antagonized. The stimulatory effect of TNF on the growth of HCs proved to be reversible and was partially blocked with either anti-TNF receptor or anti-lymphotoxin antibodies. Cellular or secreted thymidine kinase levels reflected the proliferative state of HCs in response to different in vitro treatments, as confirmed by thymidine incorporation and cell cycle studies.
AB - The effect of alpha-interferon (αIFN) on spontaneous or induced proliferation of isolated peripheral blood mononuclear cells from 5 hairy cell leukemia patients was studied. a-IFN inhibited the low spontaneous proliferation of B-1y7 positive hairy cells (HCs) and also the proliferation induced by tumour necrosis factor (TNF). Interleukin-2 did not affect HCs, but induced CD4 positive T cells to proliferate, an effect which αIFN antagonized. The stimulatory effect of TNF on the growth of HCs proved to be reversible and was partially blocked with either anti-TNF receptor or anti-lymphotoxin antibodies. Cellular or secreted thymidine kinase levels reflected the proliferative state of HCs in response to different in vitro treatments, as confirmed by thymidine incorporation and cell cycle studies.
KW - Deoxythymidine kinase
KW - Hairy cell leukemia
KW - Interferon
KW - Interleukin-2
KW - Thymidine incorporation
KW - Tumor necrosis factor
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U2 - 10.3109/10428199209053604
DO - 10.3109/10428199209053604
M3 - Article
C2 - 1472934
AN - SCOPUS:0026615752
SN - 1042-8194
VL - 7
SP - 69
EP - 77
JO - Leukemia and Lymphoma
JF - Leukemia and Lymphoma
IS - 1-2
ER -