TY - JOUR
T1 - Alterations in atrial natriuretic peptide and its receptors in streptozotocin-induced diabetic rat kidneys
AU - Obineche, Enyioma N.
AU - Adeghate, Ernest
AU - Chandranath, Irwin S.
AU - Benedict, Sheela
AU - Al Gafri, Laila S.
AU - Adem, Abdu
N1 - Funding Information:
This research was supported by a grant from the Faculty of Medicine and Health Sciences, United Arab Emirates University. The technical assistance of Kholoud Arafat, Omar Naseer, Mohamed Shafiullah and Abdul Samad Ponery is highly appreciated.
PY - 2004/6
Y1 - 2004/6
N2 - In this study the effect of diabetes mellitus on atrial natriuretic peptide (ANP) receptors in streptozotocin- (STZ-) induced diabetic rat kidneys was studied. Moreover, plasma ANP concentration was evaluated in diabetic and control rats by using radioimmunoassay. In addition, the expression of ANP in the kidneys of control and diabetic rats was evaluated by immunohistochemistry. Body-weight loss and increased glucose levels were used as indices of diabetes mellitus in the STZ-induced rats. There was a significant loss in the body weight of the diabetic rats compared to controls. The efficacy of STZ administration was confirmed by rising blood glucose levels, which were significantly higher in diabetic rats compared to controls. Plasma ANP concentration was significantly greater in the diabetic rats in comparison with controls. Moreover, our immunohistochemical results show that the expression of ANP in diabetic rats was higher than that in age-matched controls. ANP was observed in the cells lining the proximal convoluted tubules in the cortex. The distribution and levels of ANP receptors in the kidneys of diabetic rats and age-matched controls were investigated using quantitative receptor autoradiography. Our results demonstrate significant decrease in ANP receptors in the kidneys of the diabetic rats compared to controls. The significant decrease was found in the juxtaglomerular medulla, inner medulla, and the papillae. The decrease in ANP receptors observed in the diabetic kidneys could have pathological consequences resulting in renal resistance to ANP in diabetes.
AB - In this study the effect of diabetes mellitus on atrial natriuretic peptide (ANP) receptors in streptozotocin- (STZ-) induced diabetic rat kidneys was studied. Moreover, plasma ANP concentration was evaluated in diabetic and control rats by using radioimmunoassay. In addition, the expression of ANP in the kidneys of control and diabetic rats was evaluated by immunohistochemistry. Body-weight loss and increased glucose levels were used as indices of diabetes mellitus in the STZ-induced rats. There was a significant loss in the body weight of the diabetic rats compared to controls. The efficacy of STZ administration was confirmed by rising blood glucose levels, which were significantly higher in diabetic rats compared to controls. Plasma ANP concentration was significantly greater in the diabetic rats in comparison with controls. Moreover, our immunohistochemical results show that the expression of ANP in diabetic rats was higher than that in age-matched controls. ANP was observed in the cells lining the proximal convoluted tubules in the cortex. The distribution and levels of ANP receptors in the kidneys of diabetic rats and age-matched controls were investigated using quantitative receptor autoradiography. Our results demonstrate significant decrease in ANP receptors in the kidneys of the diabetic rats compared to controls. The significant decrease was found in the juxtaglomerular medulla, inner medulla, and the papillae. The decrease in ANP receptors observed in the diabetic kidneys could have pathological consequences resulting in renal resistance to ANP in diabetes.
KW - Atrial natriuretic peptide
KW - Diabetes
KW - Kidney
KW - Rat
KW - Receptors
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U2 - 10.1023/B:MCBI.0000028732.15710.c2
DO - 10.1023/B:MCBI.0000028732.15710.c2
M3 - Article
C2 - 15362480
AN - SCOPUS:3442895933
SN - 0300-8177
VL - 261
SP - 3
EP - 8
JO - Molecular and cellular biochemistry
JF - Molecular and cellular biochemistry
IS - 1
ER -