TY - JOUR
T1 - Alterations in lipid peroxidation, electrolyte leakage, and proline metabolism in Catharanthus roseus under treatment with triadimefon, a systemic fungicide
AU - Jaleel, Cheruth Abdul
AU - Gopi, Ragupathi
AU - Panneerselvam, Rajaram
PY - 2007/12
Y1 - 2007/12
N2 - Triadimefon (TDM), a systemic fungicide with non-traditional plant-growth regulator properties, was administered to Catharanthus roseus (L.) G. Don. plants in order to determine its effects on oxidative injury in terms of H2O2 content, lipid peroxidation (LPO), electrolyte leakage (EL), protein and amino acid contents, as well as proline metabolism. The LPO, estimated as thiobarbituric acid-reactive substances (TBARS), decreased under TDM treatment. It was found that H2O2 and EL were reduced under TDM treatment when compared to control. TDM treatment caused a significant increase in the protein and amino acid contents. Glycine betaine (GB) and proline (PRO) significantly accumulated in C. roseus under stress arisen from fungicide applications. Proline oxidase (PROX) activities reduce the PRO content and γ-glutamyl kinase (γ-GK) accelerates the synthesis of PRO. Under TDM treatment, the activity of PROX decreased and the γ-GK activity increased. From our results, it is suggested that fungicide triadimefon causes activation of metabolic processes in the medicinal plant Catharanthus roseus. These findings are of great significance for the cultivation of this medicinal plant, as it was previously reported that TDM causes an enhancement of antioxidant metabolism and ajmalicine production in C. roseus. To cite this article: C.A. Jaleel et al., C. R. Biologies 330 (2007).
AB - Triadimefon (TDM), a systemic fungicide with non-traditional plant-growth regulator properties, was administered to Catharanthus roseus (L.) G. Don. plants in order to determine its effects on oxidative injury in terms of H2O2 content, lipid peroxidation (LPO), electrolyte leakage (EL), protein and amino acid contents, as well as proline metabolism. The LPO, estimated as thiobarbituric acid-reactive substances (TBARS), decreased under TDM treatment. It was found that H2O2 and EL were reduced under TDM treatment when compared to control. TDM treatment caused a significant increase in the protein and amino acid contents. Glycine betaine (GB) and proline (PRO) significantly accumulated in C. roseus under stress arisen from fungicide applications. Proline oxidase (PROX) activities reduce the PRO content and γ-glutamyl kinase (γ-GK) accelerates the synthesis of PRO. Under TDM treatment, the activity of PROX decreased and the γ-GK activity increased. From our results, it is suggested that fungicide triadimefon causes activation of metabolic processes in the medicinal plant Catharanthus roseus. These findings are of great significance for the cultivation of this medicinal plant, as it was previously reported that TDM causes an enhancement of antioxidant metabolism and ajmalicine production in C. roseus. To cite this article: C.A. Jaleel et al., C. R. Biologies 330 (2007).
KW - Catharanthus roseus
KW - Electrolyte leakage
KW - Glycine betaine
KW - Lipid peroxidation
KW - Proline
KW - Proline oxidase
KW - Triadimefon
KW - γ-Glutamyl kinase
UR - http://www.scopus.com/inward/record.url?scp=36549064142&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36549064142&partnerID=8YFLogxK
U2 - 10.1016/j.crvi.2007.10.001
DO - 10.1016/j.crvi.2007.10.001
M3 - Article
C2 - 18068649
AN - SCOPUS:36549064142
SN - 1631-0691
VL - 330
SP - 905
EP - 912
JO - Comptes Rendus - Biologies
JF - Comptes Rendus - Biologies
IS - 12
ER -