A mathematical model describing the kinetics of a biospecific adsorption process (affinity chromatography) in a stirred finite bath has been numerically solved by an efficient computer program. The radial adsorption distribution of β-galactosidase onto immobilized anti-β-galactosidase for different times obtained from the present solution has been satisfactorily compared with previously published experimental data, as well as with the theoretical predictions of Chase's and Arve's models. It is also shown that the present solution is more accurate than the one obtained by Chase. The computer program used in this study is a general one that can be used for any number of solutes and for equilibrium and nonequilibrium conditions.
ASJC Scopus subject areas
- Chemical Engineering(all)
- Process Chemistry and Technology
- Filtration and Separation