TY - JOUR
T1 - An atypical member of the brevinin-1 family of antimicrobial peptides isolated from the skin of the European frog Rana dalmatina
AU - Conlon, J. Michael
AU - Seidel, Bernhard
AU - Nielsen, Per F.
N1 - Funding Information:
This work was supported by an Interdisciplinary Grant (03/12-8-03-01) and a Faculty Support Grant (NP/03/01) from the United Arab Emirates University. The authors thank Drs Mahendra Patel and Agnes Sonnevend for technical assistance in the project.
PY - 2004/2
Y1 - 2004/2
N2 - A single peptide with antimicrobial activity was extracted from the skin of the European agile frog (R. dalmatina). The primary structure of this 17 amino-acid-residue peptide (ILPLLLGKVVCAITKKC) does not immediately suggest membership of any of the previously described families of antimicrobial peptides from ranid frogs. However, if it is assumed that the peptide has undergone several residue deletions during the course of speciation, it shows sequence similarity with peptides belonging to the widely distributed brevinin-1 family, particularly those isolated from the related species Rana temporaria. The minimum inhibitory concentration of the peptide, termed brevinin-1Da, against the Gram-positive bacterium Staphylococcus aureus was 7 μM and against the Gram-negative bacterium Escherichia coli was 30 μM.
AB - A single peptide with antimicrobial activity was extracted from the skin of the European agile frog (R. dalmatina). The primary structure of this 17 amino-acid-residue peptide (ILPLLLGKVVCAITKKC) does not immediately suggest membership of any of the previously described families of antimicrobial peptides from ranid frogs. However, if it is assumed that the peptide has undergone several residue deletions during the course of speciation, it shows sequence similarity with peptides belonging to the widely distributed brevinin-1 family, particularly those isolated from the related species Rana temporaria. The minimum inhibitory concentration of the peptide, termed brevinin-1Da, against the Gram-positive bacterium Staphylococcus aureus was 7 μM and against the Gram-negative bacterium Escherichia coli was 30 μM.
KW - Antimicrobial peptide
KW - Brevinin-1
KW - Frog skin
KW - HPLC purification
KW - Temporin
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U2 - 10.1016/j.cca.2004.01.003
DO - 10.1016/j.cca.2004.01.003
M3 - Article
C2 - 15050930
AN - SCOPUS:1642351416
SN - 1532-0456
VL - 137
SP - 191
EP - 196
JO - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
IS - 2
ER -