TY - JOUR
T1 - An increase in mucosal insulin-like growth factor II content in postresectional rat intestine suggests autocrine or paracrine growth stimulation
AU - Wirén, M.
AU - Adrian, T. E.
AU - Arnelo, U.
AU - Permert, J.
AU - Staab, P.
AU - Larsson, J.
N1 - Funding Information:
We thank Minh Chu, M.D., Mrs Lena Blomqvist, and Ms Åsa Johansson for technical assistance. The IGF-I antibody was a gift from Drs. Louise Underwood and Judson J. Van Wyk, Division of Pediatric Endocrinology, University of North Carolina at Chapel Hill. This antibody is distributed by the National Institute for Diabetes, Digestive and Kidney Disorders, through the national Hormone and Pituitary Program. The EGF antibody was a gift from Drs. Andrew Garner and the late Harry Gregory, Imperial Chemical Industries Pharmaceutical Division, Alderly Park, Macclesfield, Cheshire. This study was supported by grants from The Medical Research Council (10402), County Council of Östergötland, Semper AB, and in part by the state of Nebraska Cancer and Smoking-Related Disease Program (LB 595).
PY - 1998
Y1 - 1998
N2 - Background: Luminal nutrients and growth factors regulate postresectional intestinal growth. The interplay between glutamine and regulatory gastrointestinal peptides is not known. Methods: The effects of intestinal resection on tissue and plasma concentrations of peptides were studied in 60 Sprague-Dawley rats divided into resected, transected, or unoperated groups. Subgroups were fed either a glutamine-free or a glutamine- supplemented diet for 7 days. Epidermal growth factor, transforming growth factor-α, insulin-like growth factors (IGF) I and II, peptide YY (PYY), and enteroglucagon were analyzed in intestinal mucosa and in portal plasma by radioimmunoassay. Results: No glutamine-specific effects were seen. The mucosal content of IGF-II (P < 0.01) and the portal levels of enteroglucagon and PYY (P < 0.05-0.01) increased after intestinal resection. Conclusions: The increase in PYY and enteroglucagon in portal blood supports a hormonal role in the postresectional adaptation. The increase in IGF II in the ileal mucosa, without changes in plasma, implies autocrine or paracrine growth stimulation at this stage after resection.
AB - Background: Luminal nutrients and growth factors regulate postresectional intestinal growth. The interplay between glutamine and regulatory gastrointestinal peptides is not known. Methods: The effects of intestinal resection on tissue and plasma concentrations of peptides were studied in 60 Sprague-Dawley rats divided into resected, transected, or unoperated groups. Subgroups were fed either a glutamine-free or a glutamine- supplemented diet for 7 days. Epidermal growth factor, transforming growth factor-α, insulin-like growth factors (IGF) I and II, peptide YY (PYY), and enteroglucagon were analyzed in intestinal mucosa and in portal plasma by radioimmunoassay. Results: No glutamine-specific effects were seen. The mucosal content of IGF-II (P < 0.01) and the portal levels of enteroglucagon and PYY (P < 0.05-0.01) increased after intestinal resection. Conclusions: The increase in PYY and enteroglucagon in portal blood supports a hormonal role in the postresectional adaptation. The increase in IGF II in the ileal mucosa, without changes in plasma, implies autocrine or paracrine growth stimulation at this stage after resection.
KW - Enteroglucagon
KW - Glutamine
KW - Insulin-like growth factor II
KW - Intestinal resection
KW - Peptide YY
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U2 - 10.1080/003655298750026796
DO - 10.1080/003655298750026796
M3 - Article
C2 - 9829364
AN - SCOPUS:0031732386
SN - 0036-5521
VL - 33
SP - 1080
EP - 1086
JO - Scandinavian Journal of Gastroenterology
JF - Scandinavian Journal of Gastroenterology
IS - 10
ER -