TY - JOUR
T1 - An Undergraduate Experiment
T2 - Detection of three Endocrine Disrupting Phthalates DMP, DEP and DBP in Bottled Camel Milk in the UAE
AU - Asharf, Dina
AU - Meetani, Mohammed A.
N1 - Funding Information:
This study was only made possible by the financial support of the United Arab Emirates university research office (UPAR fund # 12S090).
Publisher Copyright:
© 2023 Authors. All rights reserved.
PY - 2023
Y1 - 2023
N2 - Phthalates or esters of phthalic acid are commonly known for their extensive use as plasticizers to promote the mechanical properties of industrial plastics such as malleability, strength, softness and temperature tolerance. Since phthalates are not chemically attached to the polymers, they can freely migrate from food packages and bottles into fatty food and drinks. Phthalates are proven in several toxicological studies to be of adverse impacts on human health such as respiratory disorders, cardiovascular diseases, neurological problems, birth defects, disruption of endocrine system and different types of cancer. Camel milk is uniquely rich in fats and proteins which may interfere in the analysis of phthalates; thus, a sample preparation step is needed. Therefore, liquid-liquid extraction was performed. An analytical method using gas chromatography coupled with tandem mass spectrometry (GC-MS/MS) was developed for determinations of three phthalate esters (dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP)) in bottled camel milk samples obtained from the local markets in the UAE. Multiple reaction monitoring (MRM) mode was used for mass spectrometry detection on positive chemical ionization (PCI). Calibration curves with very good linearity were obtained for each of the three phthalates after spiking the extracted milk samples with standard concentrations of the three phthalates mixture. The analyzed milk sample was found to contain 57.6 mg.L-1 of DBP, 0.41 mg.L-1 of DMP and 0.25 mg.L-1 of DEP.
AB - Phthalates or esters of phthalic acid are commonly known for their extensive use as plasticizers to promote the mechanical properties of industrial plastics such as malleability, strength, softness and temperature tolerance. Since phthalates are not chemically attached to the polymers, they can freely migrate from food packages and bottles into fatty food and drinks. Phthalates are proven in several toxicological studies to be of adverse impacts on human health such as respiratory disorders, cardiovascular diseases, neurological problems, birth defects, disruption of endocrine system and different types of cancer. Camel milk is uniquely rich in fats and proteins which may interfere in the analysis of phthalates; thus, a sample preparation step is needed. Therefore, liquid-liquid extraction was performed. An analytical method using gas chromatography coupled with tandem mass spectrometry (GC-MS/MS) was developed for determinations of three phthalate esters (dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP)) in bottled camel milk samples obtained from the local markets in the UAE. Multiple reaction monitoring (MRM) mode was used for mass spectrometry detection on positive chemical ionization (PCI). Calibration curves with very good linearity were obtained for each of the three phthalates after spiking the extracted milk samples with standard concentrations of the three phthalates mixture. The analyzed milk sample was found to contain 57.6 mg.L-1 of DBP, 0.41 mg.L-1 of DMP and 0.25 mg.L-1 of DEP.
KW - camel milk
KW - food safety
KW - gas chromatography mass spectrometry
KW - liquid extraction
KW - phthalates
UR - http://www.scopus.com/inward/record.url?scp=85159411762&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85159411762&partnerID=8YFLogxK
U2 - 10.12691/wjce-11-2-1
DO - 10.12691/wjce-11-2-1
M3 - Article
AN - SCOPUS:85159411762
SN - 2375-1665
VL - 11
SP - 7
EP - 12
JO - World Journal of Chemical Education
JF - World Journal of Chemical Education
IS - 2
ER -
