TY - JOUR
T1 - Analysis of polarity in the expression from a multifactorial bidirectional promoter designed for high-level expression of transgenes in plants
AU - Chaturvedi, Chandra Prakash
AU - Sawant, Samir V.
AU - Kiran, Kanti
AU - Mehrotra, Rajesh
AU - Lodhi, Niraj
AU - Ansari, Suraiya Anjum
AU - Tuli, Rakesh
N1 - Funding Information:
The authors thank the Council of Scientific and Industrial Research who supported the research work under NMITLI programme of the Government of India.
PY - 2006/5/3
Y1 - 2006/5/3
N2 - A synthetic bidirectional expression module was constructed by placing a computationally designed minimal promoter sequence on the 5′ and 3′ sides of a transcription activation module. The activation of transcription from the unidirectional and bidirectional promoters constructed from the same sequence elements was evaluated by using the reporter genes gusA and gfp. The analysis based on transient and stable transformation of tobacco showed that the artificially designed multifactorial activation module activated transcription simultaneously to comparable levels in both the directions. The transcription activation module responded to elicitors like salicylic acid, NaCl and IAA in the forward as well as reverse directions. The concentration of the elicitor required for highest gene activation was similar for the two directions in case of the three activators. The kinetics of time of induction was similar in the two directions for salicylic acid and NaCl. In the case of IAA, the transcription activation was faster in the reverse direction. The results show that constitutive and chemically inducible bidirectional promoters can be deployed for predictable simultaneous regulation of two genes for genetic engineering in plants.
AB - A synthetic bidirectional expression module was constructed by placing a computationally designed minimal promoter sequence on the 5′ and 3′ sides of a transcription activation module. The activation of transcription from the unidirectional and bidirectional promoters constructed from the same sequence elements was evaluated by using the reporter genes gusA and gfp. The analysis based on transient and stable transformation of tobacco showed that the artificially designed multifactorial activation module activated transcription simultaneously to comparable levels in both the directions. The transcription activation module responded to elicitors like salicylic acid, NaCl and IAA in the forward as well as reverse directions. The concentration of the elicitor required for highest gene activation was similar for the two directions in case of the three activators. The kinetics of time of induction was similar in the two directions for salicylic acid and NaCl. In the case of IAA, the transcription activation was faster in the reverse direction. The results show that constitutive and chemically inducible bidirectional promoters can be deployed for predictable simultaneous regulation of two genes for genetic engineering in plants.
KW - Bidirectional promoter
KW - Gene expression
KW - Plant vectors
KW - Polar promoter
KW - Promoter
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U2 - 10.1016/j.jbiotec.2005.10.014
DO - 10.1016/j.jbiotec.2005.10.014
M3 - Article
C2 - 16324763
AN - SCOPUS:33646033014
SN - 0168-1656
VL - 123
SP - 1
EP - 12
JO - Journal of Biotechnology
JF - Journal of Biotechnology
IS - 1
ER -