TY - JOUR
T1 - Analysis of rate of change of IS6110 RFLP patterns of Mycobacterium tuberculosis based on serial patient isolates
AU - De Boer, Annette S.
AU - Borgdorff, Martien W.
AU - De Haas, Petra E.W.
AU - Nagelkerke, Nico J.D.
AU - Van Embden, Jan D.A.
AU - Van Soolingen, Dick
N1 - Funding Information:
Financial support: Ministry of Health, Welfare and Sports of the Netherlands; European Union (BMH4-CT97-2102).
PY - 1999
Y1 - 1999
N2 - The rate of change of IS6110 restriction fragment length polymorphism (RFLP) patterns of Mycobacterium tuberculosis was determined in serial isolates from 544 patients. In 25 patients (4.6%), the RFLP patterns of the follow-up isolates differed from the initial isolates. Patients with different follow-up strains were less likely to cluster with patients whose strains had indistinguishable RFLP patterns. Changes in RFLP patterns were more common for persons with extrapulmonary disease and for those who had both pulmonary and extrapulmonary isolates. Based on serial isolates spanning for the most part <3 months, the half-life was extrapolated to be 3.2 years (95% confidence interval, 2.1-5.0). The main implication of this study is that the rate of change of IS6110-based RFLP of M. tuberculosis supports the use of IS6110 typing in epidemiologic studies of recent transmission of tuberculosis.
AB - The rate of change of IS6110 restriction fragment length polymorphism (RFLP) patterns of Mycobacterium tuberculosis was determined in serial isolates from 544 patients. In 25 patients (4.6%), the RFLP patterns of the follow-up isolates differed from the initial isolates. Patients with different follow-up strains were less likely to cluster with patients whose strains had indistinguishable RFLP patterns. Changes in RFLP patterns were more common for persons with extrapulmonary disease and for those who had both pulmonary and extrapulmonary isolates. Based on serial isolates spanning for the most part <3 months, the half-life was extrapolated to be 3.2 years (95% confidence interval, 2.1-5.0). The main implication of this study is that the rate of change of IS6110-based RFLP of M. tuberculosis supports the use of IS6110 typing in epidemiologic studies of recent transmission of tuberculosis.
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U2 - 10.1086/314979
DO - 10.1086/314979
M3 - Article
C2 - 10479153
AN - SCOPUS:0033375318
SN - 0022-1899
VL - 180
SP - 1238
EP - 1244
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -