TY - JOUR
T1 - Anticancer Activity of Moringa peregrina (Forssk.) Fiori.
T2 - A Native Plant in Traditional Herbal Medicine of the United Arab Emirates
AU - Kaabi, Salama Khamis Sultan Al
AU - Senthilkumar, Annadurai
AU - Kizhakkayil, Jaleel
AU - Alyafei, Mohammed Abdul Muhsen
AU - Kurup, Shyam Sreedhara
AU - Dhaheri, Ayesha S.Al
AU - Jaleel, Abdul
N1 - Funding Information:
This research was funded by UAEU, UPAR#31F092.The authors would like to thank the support from MS Horticulture Program. The assistance from staff of Al Foah Experimental Station and E3, F1 labs, CAVM, UAEU is greatly acknowledged.
Funding Information:
Funding: This research was funded by UAEU, UPAR#31F092.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/1
Y1 - 2022/1
N2 - Moringa peregrina (Forssk.) Fiori. is a native desert tree growing in United Arab Emirates (UAE). The plant is being cultivated in many parts of UAE, owing to its uses in traditional medicinal and food systems. In the present study bioactivities of cultivated M. peregrina species samples are evaluated with cytotoxic studies in the human breast cancer cell line (MCF-7) and human colon adenocarcinoma cell line (Caco-2). Different extracts with hexane, chloroform, acetone and methanol were prepared from tubers, leaves and stem of M. peregrina for estimating their antioxidant contents and anticancer activities. The study was performed at different concentrations and all the extracts showed dose-depended response on both the cell lines. Among the extracts tested, the chloroform extract of stem showed remarkable anti-proliferative/cell death activity (IC50 = 45.53 µg/mL of 48 h incubation and 33.32 µg/mL of 72 h incubation) on MCF-7 cell lines. Whereas the same extract showed comparatively less activity (IC50 = 93.75 µg/mL of 48 h incubation and 87.76 µg/mL of 72 h incubation) on Caco-2 cell lines. The anti-proliferative effect of leaf extract with chloroform showed a drastic change in cell viability from 48 to 72 h incubation, in MCF-7 cells 220 to 87.5 µg/mL and in Caco-2 cells 500.9 to 72.9 µg/mL, respectively. Moreover, less than 200 µg/mL of IC50 values reported in hexane extracts of tubers (188.6 µg/mL for 48 h and 164.3 µg/mL for 72 h), acetone extracts of tubers (167.4 µg/mL for 72 h) and acetone extracts of stem (171.5 µg/mL for 48 h and 101.7 µg/mL for 72 h) on MCF-7 cells. PARP (Poly (ADP-ribose) polymerase) cleavage assay and DNA fragmentation assay performed to understand the cause of cell death. Treatment of extract on the normal fibroblast cell line required more concentration for cytotoxicity compared to the treatment on the cancer cells. This ability of the extract proved the anti-cancer property of the M. peregrina extract from the stem, tuber and leaves. The information provided in the present study enables further studies on the isolation and characterization of an anticancer molecule from the tubers of M. peregrina.
AB - Moringa peregrina (Forssk.) Fiori. is a native desert tree growing in United Arab Emirates (UAE). The plant is being cultivated in many parts of UAE, owing to its uses in traditional medicinal and food systems. In the present study bioactivities of cultivated M. peregrina species samples are evaluated with cytotoxic studies in the human breast cancer cell line (MCF-7) and human colon adenocarcinoma cell line (Caco-2). Different extracts with hexane, chloroform, acetone and methanol were prepared from tubers, leaves and stem of M. peregrina for estimating their antioxidant contents and anticancer activities. The study was performed at different concentrations and all the extracts showed dose-depended response on both the cell lines. Among the extracts tested, the chloroform extract of stem showed remarkable anti-proliferative/cell death activity (IC50 = 45.53 µg/mL of 48 h incubation and 33.32 µg/mL of 72 h incubation) on MCF-7 cell lines. Whereas the same extract showed comparatively less activity (IC50 = 93.75 µg/mL of 48 h incubation and 87.76 µg/mL of 72 h incubation) on Caco-2 cell lines. The anti-proliferative effect of leaf extract with chloroform showed a drastic change in cell viability from 48 to 72 h incubation, in MCF-7 cells 220 to 87.5 µg/mL and in Caco-2 cells 500.9 to 72.9 µg/mL, respectively. Moreover, less than 200 µg/mL of IC50 values reported in hexane extracts of tubers (188.6 µg/mL for 48 h and 164.3 µg/mL for 72 h), acetone extracts of tubers (167.4 µg/mL for 72 h) and acetone extracts of stem (171.5 µg/mL for 48 h and 101.7 µg/mL for 72 h) on MCF-7 cells. PARP (Poly (ADP-ribose) polymerase) cleavage assay and DNA fragmentation assay performed to understand the cause of cell death. Treatment of extract on the normal fibroblast cell line required more concentration for cytotoxicity compared to the treatment on the cancer cells. This ability of the extract proved the anti-cancer property of the M. peregrina extract from the stem, tuber and leaves. The information provided in the present study enables further studies on the isolation and characterization of an anticancer molecule from the tubers of M. peregrina.
KW - Breast cancer
KW - Cancer management
KW - Colon cancer
KW - Moringa peregrina
KW - Natural products
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U2 - 10.3390/horticulturae8010037
DO - 10.3390/horticulturae8010037
M3 - Article
AN - SCOPUS:85122802371
SN - 2311-7524
VL - 8
JO - Horticulturae
JF - Horticulturae
IS - 1
M1 - 37
ER -