TY - JOUR
T1 - Antimicrobial peptides of the brevinin-2 family isolated from gastric tissue of the frog, Rana esculenta
AU - Wang, Yuqi
AU - Knoop, Floyd C.
AU - Remy-Jouet, Isabelle
AU - Delarue, Catherine
AU - Vaudry, Hubert
AU - Conlon, J. Michael
N1 - Funding Information:
We thank Dr. Donald Babin, Creighton University, for amino acid analyses and Dr. Martina O’Flaherty, Creighton University, for mass spectrometry measurements. This work was supported by a grant from the National Science Foundation EPSCOR program.
PY - 1998/12/30
Y1 - 1998/12/30
N2 - Four structurally related peptides with potent growth-inhibitory activity towards Escherichia coli were isolated from an extract of the stomach of the European green frog Rana esculenta, and were identified as members of the brevinin-2 family. Two peptides, termed brevinin-2Eg (GIMDTLKNLA10 KTAGKGALQS20 LLNHASCK LS30GQC) and brevinin-2Eh (GIMDTLKNLA10 KTAGKGALQS20 LLNHASCKL S30 KQC) have not been described previously. One peptide is identical to brevinin-2Ec, previously isolated from R. esculenta skin secretions, and one peptide is identical to brevinin-2Ef whose structure has been deduced from a cloned cDNA prepared from a R. esculenta skin cDNA library. The data demonstrate that certain peptides of the brevinin-2 family, like the magainins in the toad, Xenopus laevis, may play an important role in protecting the gastrointestinal tract of Ranid frogs against microbial invasion.
AB - Four structurally related peptides with potent growth-inhibitory activity towards Escherichia coli were isolated from an extract of the stomach of the European green frog Rana esculenta, and were identified as members of the brevinin-2 family. Two peptides, termed brevinin-2Eg (GIMDTLKNLA10 KTAGKGALQS20 LLNHASCK LS30GQC) and brevinin-2Eh (GIMDTLKNLA10 KTAGKGALQS20 LLNHASCKL S30 KQC) have not been described previously. One peptide is identical to brevinin-2Ec, previously isolated from R. esculenta skin secretions, and one peptide is identical to brevinin-2Ef whose structure has been deduced from a cloned cDNA prepared from a R. esculenta skin cDNA library. The data demonstrate that certain peptides of the brevinin-2 family, like the magainins in the toad, Xenopus laevis, may play an important role in protecting the gastrointestinal tract of Ranid frogs against microbial invasion.
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U2 - 10.1006/bbrc.1998.9822
DO - 10.1006/bbrc.1998.9822
M3 - Article
C2 - 9918774
AN - SCOPUS:0032583460
SN - 0006-291X
VL - 253
SP - 600
EP - 603
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -