Antimicrobial peptides with therapeutic potential from skin secretions of the Marsabit clawed frog Xenopus borealis (Pipidae)

Milena Mechkarska, Eman Ahmed, Laurent Coquet, Jérôme Leprince, Thierry Jouenne, Hubert Vaudry, Jay D. King, J. Michael Conlon

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Nine peptides with differential growth inhibitory activity against Escherichia coli and Staphylococcus aureus were isolated from norepinephrine-stimulated skin secretions of the tetraploid frog Xenopus borealis Parker, 1936 (Pipidae). Structural characterization of the peptides demonstrated that they were orthologous to magainin-2 (1 peptide), peptide glycine-leucine-amide, PGLa (2 peptides), caerulein-precursor fragments, CPF (4 peptides), and xenopsin-precursor fragments, XPF (2 peptides), previously isolated from Xenopus laevis and X. amieti. In addition, a second magainin-related peptide (G**KFLHSAGKFGKAFLGEVMIG) containing a two amino acid residue deletion compared with magainin-2 was identified that had only weak antimicrobial activity. The peptide with the greatest potential for development into a therapeutically valuable anti-infective agent was CPF-B1 (GLGSLLGKAFKIGLKTVGKMMGGAPREQ) with MIC=5μM against E. coli, MIC=5μM against S. aureus, and MIC=25μM against Candida albicans, and low hemolytic activity against human erythrocytes (LC50>200μM). This peptide was also the most abundant antimicrobial peptide in the skin secretions. CPF-B1 was active against clinical isolates of the nosocomial pathogens, methicillin-resistant S. aureus (MRSA) and multidrug-resistant Acinetobacter baumannii (MDRAB) with MIC values in the range 4-8μM.

Original languageEnglish
Pages (from-to)467-472
Number of pages6
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume152
Issue number4
DOIs
Publication statusPublished - Nov 2010
Externally publishedYes

Keywords

  • Antimicrobial peptide
  • Frog skin
  • Magainin
  • PGLa
  • Procaerulein
  • Proxenopsin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Toxicology
  • Cell Biology
  • Health, Toxicology and Mutagenesis

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