Antioxidant activity, lipoxygenase inhibitory effect and polyphenolic compounds from Calotropis procera (Ait.) R. Br

Mohammad Ahmad Khasawneh, Hanan Mohamed Elwy, Nael Mohamed Fawzi, Alaaeldin Ahmed Hamza, Abdul Raheem Chevidenkandy, Ahmed H. Hassan

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


The purpose for this study was to evaluate the biological, antioxidant, anti-inflammatory and anti-cancer properties of Calotropis procera alcoholic extract and its various solvent sub fractions. The total antioxidant property was estimated by the Ferric Reducing Antioxidant Power (FRAP), l,l-diphenyl-2-picrylhydrazyl (DPPH·), 2, 2-azino-bis (3-ethylbenzothiazoline-6-sulfonate) (ABTS·+) and β-carotene bleaching tests. Among the ethanol extract and three fractions, the ethyl acetate fraction showed the highest phenolic content (108.26 mg gallic acid g -1) and the best antioxidant activity. The ascorbic acid equivalent antioxidant capacities of the ethyl acetate fraction were 0.26, 0.53, 0.17 mmol g -1 in FRAP, ABTS and DPPH assays, respectively. This study verified that the ethanol extract and ethyl acetate fraction from Calotropis procera have strong antioxidant activity that were correlated their high level of phenolic content. Furthermore, the same extract showed appreciable anti-inflammatory via lipoxygenase inhibitory activity (LOX), IC50 values ranged from 7.12-52.68 (μg mL -1. Moreover, ethyl acetate fraction showed the strongest cytotoxic effect (IC 50 = 10.6 μg mL -1) against MCF-7 breast cancer cell line.

Original languageEnglish
Pages (from-to)80-88
Number of pages9
JournalResearch Journal of Phytochemistry
Issue number2
Publication statusPublished - 2011


  • Anticancer activity
  • Antioxidant activity
  • Calotropis procera
  • Lipoxygenase inhibitory effects
  • MTT assay
  • Medicinal plants
  • Polyphenolic compounds

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Plant Science


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