TY - JOUR
T1 - Antioxidant activity, lipoxygenase inhibitory effect and polyphenolic compounds from Calotropis procera (Ait.) R. Br
AU - Khasawneh, Mohammad Ahmad
AU - Elwy, Hanan Mohamed
AU - Fawzi, Nael Mohamed
AU - Hamza, Alaaeldin Ahmed
AU - Chevidenkandy, Abdul Raheem
AU - Hassan, Ahmed H.
PY - 2011
Y1 - 2011
N2 - The purpose for this study was to evaluate the biological, antioxidant, anti-inflammatory and anti-cancer properties of Calotropis procera alcoholic extract and its various solvent sub fractions. The total antioxidant property was estimated by the Ferric Reducing Antioxidant Power (FRAP), l,l-diphenyl-2-picrylhydrazyl (DPPH·), 2, 2-azino-bis (3-ethylbenzothiazoline-6-sulfonate) (ABTS·+) and β-carotene bleaching tests. Among the ethanol extract and three fractions, the ethyl acetate fraction showed the highest phenolic content (108.26 mg gallic acid g -1) and the best antioxidant activity. The ascorbic acid equivalent antioxidant capacities of the ethyl acetate fraction were 0.26, 0.53, 0.17 mmol g -1 in FRAP, ABTS and DPPH assays, respectively. This study verified that the ethanol extract and ethyl acetate fraction from Calotropis procera have strong antioxidant activity that were correlated their high level of phenolic content. Furthermore, the same extract showed appreciable anti-inflammatory via lipoxygenase inhibitory activity (LOX), IC50 values ranged from 7.12-52.68 (μg mL -1. Moreover, ethyl acetate fraction showed the strongest cytotoxic effect (IC 50 = 10.6 μg mL -1) against MCF-7 breast cancer cell line.
AB - The purpose for this study was to evaluate the biological, antioxidant, anti-inflammatory and anti-cancer properties of Calotropis procera alcoholic extract and its various solvent sub fractions. The total antioxidant property was estimated by the Ferric Reducing Antioxidant Power (FRAP), l,l-diphenyl-2-picrylhydrazyl (DPPH·), 2, 2-azino-bis (3-ethylbenzothiazoline-6-sulfonate) (ABTS·+) and β-carotene bleaching tests. Among the ethanol extract and three fractions, the ethyl acetate fraction showed the highest phenolic content (108.26 mg gallic acid g -1) and the best antioxidant activity. The ascorbic acid equivalent antioxidant capacities of the ethyl acetate fraction were 0.26, 0.53, 0.17 mmol g -1 in FRAP, ABTS and DPPH assays, respectively. This study verified that the ethanol extract and ethyl acetate fraction from Calotropis procera have strong antioxidant activity that were correlated their high level of phenolic content. Furthermore, the same extract showed appreciable anti-inflammatory via lipoxygenase inhibitory activity (LOX), IC50 values ranged from 7.12-52.68 (μg mL -1. Moreover, ethyl acetate fraction showed the strongest cytotoxic effect (IC 50 = 10.6 μg mL -1) against MCF-7 breast cancer cell line.
KW - Anticancer activity
KW - Antioxidant activity
KW - Calotropis procera
KW - Lipoxygenase inhibitory effects
KW - MTT assay
KW - Medicinal plants
KW - Polyphenolic compounds
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U2 - 10.3923/rjphyto.2011.80.88
DO - 10.3923/rjphyto.2011.80.88
M3 - Article
AN - SCOPUS:80055064656
SN - 1819-3471
VL - 5
SP - 80
EP - 88
JO - Research Journal of Phytochemistry
JF - Research Journal of Phytochemistry
IS - 2
ER -