Antioxidant, anti-lipoxygenase and cytotoxic activity of leptadenia pyrotechnica (Forssk.) decne polyphenolic constituents

Mohammad A. Khasawneh, Hanan M. Elwy, Alaaeldin A. Hamza, Nael M. Fawzi, Ahmed H. Hassan

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Leptadenia pyrotechnica Forssk is a traditional medicinal herb used for treatment of inflammatory diseases and cancer. In this research, the aqueous ethanolic crude extract of Leptadenia pyrotechnica aerial parts, along with its ethyl acetate, n-butanol and water partitioning fractions were evaluated for their antioxidant capacity, polyphenolic content, anti-inflammatory and anti-cancer properties. The total antioxidant capacity was estimated by the FRAP, DPPH, ABTS and β-carotene bleaching assays.The ethyl acetate fraction exhibited the highest polyphenolic content (252.27 mg gallic acid/g) and the best antioxidant activity (1.2, 0.57, 0.45 mmol ascorbic acid equivalent/g in the FRAP, ABTS and DPPH assays, respectively). Furthermore, the same extract showed appreciable anti-inflammatory via lipoxygenase (LOX) inhibitory activity (IC50 = 1.41 μg/mL). Moreover, the ethyl acetate fraction also showed the strongest cytotoxic effect (IC50 = 43.16 μg/mL) against MCF-7 human breast cancer cell line. These results suggest that this plant may be considered an interesting source of compounds with antioxidant, anti-inflammatory and anti-cancer properties for therapeutic, nutraceutical and functional food applications.

Original languageEnglish
Pages (from-to)7510-7521
Number of pages12
JournalMolecules
Volume16
Issue number9
DOIs
Publication statusPublished - Sep 2011

Keywords

  • Anti-inflammatory
  • Antioxidant
  • Cytotoxicity
  • Leptadenia pyrotechnica
  • Total phenol

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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