Antiproliferative potential of pergularia daemia (forsk.) on human oral epidermoid carcinoma (kb) cells by inducing apoptosis and modifying oxidant antioxidant status

Methods: Different concentrations of areal part of P. daemia methanolic extract (PDME) (10, 20, 40, 80, 160, 320 μg/mL) were subjected to cytotoxic study. The antiproliferative effect of PDME was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, analysis of reactive oxygen species (ROS) generation, mitochondrial membrane potential, cell cycle arrest, and antioxidant status.

Result: Increased level of intracellular ROS, lipid peroxidation marker (Thiobarbituric acid reactive substances), DNA damage (comet assay), apoptotic death and cell cycle arrest in PDME treated cells. Whereas decreased activity of antioxidants and altered mitochondrial membrane potential were observed in PDME treated cells.

Conclusion: The current investigation suggested that the phyto constituents of P. daemia responsible for anticancer activity. Thus, the long-term consumption of P. daemia could be considered and promoted as an adjuvant therapy for various malignancy.

Objective: Management of cancer without any side-effects is still a challenge for the medicinal system. This leads to an increasing search for improved anticancer drugs. Few of plant products have been used in traditional medicine for thousands of years and have been drawing of great deal of attention to suppress cancer. The main objective of this study is to evaluate the anti-proliferative effect of Pergularia daemia (Forsk) against KB cells.