Beyond Genome Editing: CRISPR Approaches

Qaisar Sultan, Sidra Ashraf, Ahmad Munir, Sultan Habibullah Khan, Nayla Munawar, Kamel A. Abd-Elsalam, Aftab Ahmad

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)


CRISPR has become a powerful tool for precise editing of user-defined genomic loci in living organisms. Within mere eight years, CRISPR/Cas has become an indispensable tool in fundamental and applied research of biological sciences. Today CRISPR is no longer just a method to edit a specific locus, but applications of dCas9-based platforms are exceeding the genome editing functions of WT Cas9. Applications of CRISPR technology are extending beyond genome editing such as expression modulation, rewriting epigenetic signatures, and imaging spatiotemporal organization of the genome. In this chapter we discuss, dCas9 and engineered gRNA-based CRISPRi and CRISPRa approach for transcriptional regulation of a gene. In addition, we also describe visualization of spatiotemporal organization of genomic loci through dCas9-based tools. Moreover, we also explain how CRISPR barcodes are exploited to study cell lineage. Furthermore, we also highlight CRISPR-mediated tools such as epigenome regulation, base editing, prime editing, and directed evolution. Finally, we discussed prospects beyond genome editing application of CRISPR/Cas.

Original languageEnglish
Title of host publicationThe CRISPR/Cas Tool Kit for Genome Editing
PublisherSpringer Nature
Number of pages32
ISBN (Electronic)9789811663055
ISBN (Print)9789811663048
Publication statusPublished - Jan 1 2022


  • Base editing
  • Beyond genome editing
  • CRISPR/Cas
  • EvolvR
  • Gene drive
  • Genome imaging
  • Prime editing

ASJC Scopus subject areas

  • General Medicine
  • General Health Professions


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