Bioactive properties and gut microbiota modulation by date seed polysaccharides extracted using ultrasound-assisted deep eutectic solvent

Athira Jayasree Subhash, Gafar Babatunde Bamigbade, Mohammed Tarique, Basel Al-Ramadi, Basim Abu-Jdayil, Afaf Kamal-Eldin, Laura Nyström, Mutamed Ayyash

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Polysaccharides are abundant macromolecules. The study extracted date seed polysaccharides (UPS) using ultrasound-assisted deep eutectic solvent extraction to valorize date seeds. UPS were subjected to comprehensive characterization and evaluation of their bioactivity, prebiotic properties, and their potential to modulate the gut microbiome. Characterization revealed UPS's heteropolysaccharide composition with galactose, mannose, fructose, glucose, and galacturonic acid respectively in 66.1, 13.3, 9.9, 5.4, and 5.1%. UPS showed a concentration-dependent increase of radical scavenging and antioxidant activities, evidenced by FRAP, TAC, and RP assays. They also displayed antimicrobial efficacy against E. coli O157:H7, S. typhimurium, S. aureus, and L. monocytogenes. Rheological analysis showed UPS's elastic-dominant nature with thixotropic tendencies. UPS inhibited α-glycosidase, α-amylase, and ACE up to 86%, and reduced Caco-2 and MCF-7 cell viability by 70% and 46%, respectively. UPS favored beneficial gut microbiota growth, releasing significant SCFAs during fecal fermentation.

Original languageEnglish
Article number101354
JournalFood Chemistry: X
Volume22
DOIs
Publication statusPublished - Jun 30 2024

Keywords

  • Gut microbiome
  • Prebiotic
  • Probiotic
  • Ultrasound polysaccharides

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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