TY - JOUR
T1 - Bioactive properties and gut microbiota modulation by date seed polysaccharides extracted using ultrasound-assisted deep eutectic solvent
AU - Subhash, Athira Jayasree
AU - Bamigbade, Gafar Babatunde
AU - Tarique, Mohammed
AU - Al-Ramadi, Basel
AU - Abu-Jdayil, Basim
AU - Kamal-Eldin, Afaf
AU - Nyström, Laura
AU - Ayyash, Mutamed
N1 - Publisher Copyright:
© 2024 The Author(s)
PY - 2024/6/30
Y1 - 2024/6/30
N2 - Polysaccharides are abundant macromolecules. The study extracted date seed polysaccharides (UPS) using ultrasound-assisted deep eutectic solvent extraction to valorize date seeds. UPS were subjected to comprehensive characterization and evaluation of their bioactivity, prebiotic properties, and their potential to modulate the gut microbiome. Characterization revealed UPS's heteropolysaccharide composition with galactose, mannose, fructose, glucose, and galacturonic acid respectively in 66.1, 13.3, 9.9, 5.4, and 5.1%. UPS showed a concentration-dependent increase of radical scavenging and antioxidant activities, evidenced by FRAP, TAC, and RP assays. They also displayed antimicrobial efficacy against E. coli O157:H7, S. typhimurium, S. aureus, and L. monocytogenes. Rheological analysis showed UPS's elastic-dominant nature with thixotropic tendencies. UPS inhibited α-glycosidase, α-amylase, and ACE up to 86%, and reduced Caco-2 and MCF-7 cell viability by 70% and 46%, respectively. UPS favored beneficial gut microbiota growth, releasing significant SCFAs during fecal fermentation.
AB - Polysaccharides are abundant macromolecules. The study extracted date seed polysaccharides (UPS) using ultrasound-assisted deep eutectic solvent extraction to valorize date seeds. UPS were subjected to comprehensive characterization and evaluation of their bioactivity, prebiotic properties, and their potential to modulate the gut microbiome. Characterization revealed UPS's heteropolysaccharide composition with galactose, mannose, fructose, glucose, and galacturonic acid respectively in 66.1, 13.3, 9.9, 5.4, and 5.1%. UPS showed a concentration-dependent increase of radical scavenging and antioxidant activities, evidenced by FRAP, TAC, and RP assays. They also displayed antimicrobial efficacy against E. coli O157:H7, S. typhimurium, S. aureus, and L. monocytogenes. Rheological analysis showed UPS's elastic-dominant nature with thixotropic tendencies. UPS inhibited α-glycosidase, α-amylase, and ACE up to 86%, and reduced Caco-2 and MCF-7 cell viability by 70% and 46%, respectively. UPS favored beneficial gut microbiota growth, releasing significant SCFAs during fecal fermentation.
KW - Gut microbiome
KW - Prebiotic
KW - Probiotic
KW - Ultrasound polysaccharides
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UR - http://www.scopus.com/inward/citedby.url?scp=85189755282&partnerID=8YFLogxK
U2 - 10.1016/j.fochx.2024.101354
DO - 10.1016/j.fochx.2024.101354
M3 - Article
AN - SCOPUS:85189755282
SN - 2590-1575
VL - 22
JO - Food Chemistry: X
JF - Food Chemistry: X
M1 - 101354
ER -