TY - JOUR
T1 - Biodegradation of fuel oxygenates by sol-gel immobilized bacteria Aquincola tertiaricarbonis L108
AU - Pannier, Angela
AU - Oehm, Claudia
AU - Fischer, Axel R.
AU - Werner, Peter
AU - Soltmann, Ulrich
AU - Böttcher, Horst
N1 - Funding Information:
This study was supported by the German federal Ministry of Education and Research (BMBF project NANOKAT, Grant No. 02WR0696 ). The authors would like to thank the Helmholtz Center for Environmental Research (UFZ, Leipzig, Germany) for kindly providing the bacterial strain Aquincola tertiaricarbonis L108. Furthermore, we are particularly grateful to Dr. Hofinger (Namos, Dresden) for his encouragement and advices concerning the cultivation of the bacterial strain.
PY - 2010/11/8
Y1 - 2010/11/8
N2 - The development of long-term storable biofilters containing bacteria which are capable to degrade recalcitrant environmental compounds like the fuel oxygenates methyl tert-butyl ether (MTBE) and ethyl tert-butyl ether (ETBE) is of special interest for the treatment of contaminated water. Fuel oxygenates have been proven to be more persistent to biodegradation than other gasoline components. Only a few microorganisms, e.g. Aquincola tertiaricarbonis L108, are capable to biodegrade these substances.In order to develop highly efficient and long-term stable biocomposite materials for bioremediation, the applicability of sol-gel matrices for the immobilization of A. tertiaricarbonis was tested. Two different sol-gel immobilization techniques were used: (A) the immobilization within ceramic-like shapes by using the freeze-gelation technique and (B) sol-gel coatings on an inert, porous material. For comparison, the widely used alginate-bead immobilization technique has been applied.The embedding of A. tertiaricarbonis within freeze-gelation composites (method A) proved to be not applicable because this strain was too sensitive regarding the applied freezing and drying conditions even though cell-protecting additives were added. In contrast, A. tertiaricarbonis immobilized on porous expanded clay pellets (method B) could be stored under humid atmosphere at least for a time of 8 months without a significant reduction of their metabolic activity.
AB - The development of long-term storable biofilters containing bacteria which are capable to degrade recalcitrant environmental compounds like the fuel oxygenates methyl tert-butyl ether (MTBE) and ethyl tert-butyl ether (ETBE) is of special interest for the treatment of contaminated water. Fuel oxygenates have been proven to be more persistent to biodegradation than other gasoline components. Only a few microorganisms, e.g. Aquincola tertiaricarbonis L108, are capable to biodegrade these substances.In order to develop highly efficient and long-term stable biocomposite materials for bioremediation, the applicability of sol-gel matrices for the immobilization of A. tertiaricarbonis was tested. Two different sol-gel immobilization techniques were used: (A) the immobilization within ceramic-like shapes by using the freeze-gelation technique and (B) sol-gel coatings on an inert, porous material. For comparison, the widely used alginate-bead immobilization technique has been applied.The embedding of A. tertiaricarbonis within freeze-gelation composites (method A) proved to be not applicable because this strain was too sensitive regarding the applied freezing and drying conditions even though cell-protecting additives were added. In contrast, A. tertiaricarbonis immobilized on porous expanded clay pellets (method B) could be stored under humid atmosphere at least for a time of 8 months without a significant reduction of their metabolic activity.
KW - Alginate beads
KW - Aquincola tertiaricarbonis
KW - Expanded clay pellets
KW - Freeze-gelation biocers
KW - Methyl tert-butyl ether
KW - Sol-gel immobilization
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U2 - 10.1016/j.enzmictec.2010.07.014
DO - 10.1016/j.enzmictec.2010.07.014
M3 - Article
AN - SCOPUS:77956483017
SN - 0141-0229
VL - 47
SP - 291
EP - 296
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
IS - 6
ER -