Biosynthesis of tachykinins (substance P, neurokinin A and neuropeptide K) in neurons of the guinea pig myenteric plexus

Carolyn F. Deacon; J. Michael Conlon

doi:10.1016/0197-0186(87)90085-4

Biosynthesis of tachykinins (substance P, neurokinin A and neuropeptide K) in neurons of the guinea pig myenteric plexus

Carolyn F. Deacon, J. Michael Conlon

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Incubation of the myenteric plexus-containing longitudinal muscle layer of guinea pig small intestine with [³⁵S]methionine for up to 9 h resulted in a progressive increase in the incorporation of radioactivity into newly synthesized substance P, neurokinin A and neuropeptide K. The radiolabelled peptides were isolated from tissue extracts by immunoprecipitation using regionally-specific antisera and purified by reverse-phase high performance liquid chromatography. Incorporation of radioactivity into the tachykinins was abolished by cycloheximide and was not observed when [³⁵S]cysteine was substituted for [³⁵S]methionine. The method may be used to study the regulation of biosynthesis and posttranslational modification of protachykinins in the gut.

Original language	English
Pages (from-to)	559-564
Number of pages	6
Journal	Neurochemistry International
Volume	10
Issue number	4
DOIs	https://doi.org/10.1016/0197-0186(87)90085-4
Publication status	Published - 1987
Externally published	Yes

ASJC Scopus subject areas

Cellular and Molecular Neuroscience
Cell Biology

Access to Document

10.1016/0197-0186(87)90085-4

Cite this

@article{da212e10250f49f2aa8c67b91cabe3ec,

title = "Biosynthesis of tachykinins (substance P, neurokinin A and neuropeptide K) in neurons of the guinea pig myenteric plexus",

abstract = "Incubation of the myenteric plexus-containing longitudinal muscle layer of guinea pig small intestine with [35S]methionine for up to 9 h resulted in a progressive increase in the incorporation of radioactivity into newly synthesized substance P, neurokinin A and neuropeptide K. The radiolabelled peptides were isolated from tissue extracts by immunoprecipitation using regionally-specific antisera and purified by reverse-phase high performance liquid chromatography. Incorporation of radioactivity into the tachykinins was abolished by cycloheximide and was not observed when [35S]cysteine was substituted for [35S]methionine. The method may be used to study the regulation of biosynthesis and posttranslational modification of protachykinins in the gut.",

author = "Deacon, {Carolyn F.} and Conlon, {J. Michael}",

note = "Funding Information: was supported by the",

year = "1987",

doi = "10.1016/0197-0186(87)90085-4",

language = "English",

volume = "10",

pages = "559--564",

journal = "Neurochemistry International",

issn = "0197-0186",

publisher = "Elsevier Limited",

number = "4",

}

TY - JOUR

T1 - Biosynthesis of tachykinins (substance P, neurokinin A and neuropeptide K) in neurons of the guinea pig myenteric plexus

AU - Deacon, Carolyn F.

AU - Conlon, J. Michael

N1 - Funding Information: was supported by the

PY - 1987

Y1 - 1987

N2 - Incubation of the myenteric plexus-containing longitudinal muscle layer of guinea pig small intestine with [35S]methionine for up to 9 h resulted in a progressive increase in the incorporation of radioactivity into newly synthesized substance P, neurokinin A and neuropeptide K. The radiolabelled peptides were isolated from tissue extracts by immunoprecipitation using regionally-specific antisera and purified by reverse-phase high performance liquid chromatography. Incorporation of radioactivity into the tachykinins was abolished by cycloheximide and was not observed when [35S]cysteine was substituted for [35S]methionine. The method may be used to study the regulation of biosynthesis and posttranslational modification of protachykinins in the gut.

AB - Incubation of the myenteric plexus-containing longitudinal muscle layer of guinea pig small intestine with [35S]methionine for up to 9 h resulted in a progressive increase in the incorporation of radioactivity into newly synthesized substance P, neurokinin A and neuropeptide K. The radiolabelled peptides were isolated from tissue extracts by immunoprecipitation using regionally-specific antisera and purified by reverse-phase high performance liquid chromatography. Incorporation of radioactivity into the tachykinins was abolished by cycloheximide and was not observed when [35S]cysteine was substituted for [35S]methionine. The method may be used to study the regulation of biosynthesis and posttranslational modification of protachykinins in the gut.

UR - http://www.scopus.com/inward/record.url?scp=45949125636&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=45949125636&partnerID=8YFLogxK

U2 - 10.1016/0197-0186(87)90085-4

DO - 10.1016/0197-0186(87)90085-4

M3 - Article

AN - SCOPUS:45949125636

SN - 0197-0186

VL - 10

SP - 559

EP - 564

JO - Neurochemistry International

JF - Neurochemistry International

IS - 4

ER -

Biosynthesis of tachykinins (substance P, neurokinin A and neuropeptide K) in neurons of the guinea pig myenteric plexus

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this