Brain parenchymal TNF-α and IL-1β induction in experimental pneumococcal meningitis

Kaywan Izadpanah; Dorette Freyer; Joerg R. Weber; Johann S. Braun

doi:10.1016/j.jneuroim.2014.08.625

Brain parenchymal TNF-α and IL-1β induction in experimental pneumococcal meningitis

Kaywan Izadpanah, Dorette Freyer, Joerg R. Weber, Johann S. Braun

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Triggers of brain inflammation in pneumococcal meningitis are unknown. TNF-α and IL-1β were upregulated (real time PCR and in situ hybridization) in neurons and astrocytes time-dependently and maximally in the hippocampus during murine pneumococcal meningitis. Upregulation of TNF-α and IL-1β mRNA in the brain parenchyma was independent of cerebrospinal fluid leukocytosis, pneumococcal pneumolysin and H₂O₂, but it was potently induced by pneumococcal cell wall (PCW) fragments. Brain TNF-α mRNA was downregulated by a matrix metalloproteinases inhibitor. PCW fragments were located in the brain parenchyma. In conclusion, PCW fragments and matrix metalloproteinases trigger cytokine induction in the brain parenchyma during pneumococcal meningitis.

Original language	English
Pages (from-to)	104-111
Number of pages	8
Journal	Journal of Neuroimmunology
Volume	276
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2014.08.625
Publication status	Published - Nov 15 2014
Externally published	Yes

Keywords

Brain
IL-1β
Meningitis
Pneumococcal cell wall
S. pneumoniae
TNF-α

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/j.jneuroim.2014.08.625

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title = "Brain parenchymal TNF-α and IL-1β induction in experimental pneumococcal meningitis",

abstract = "Triggers of brain inflammation in pneumococcal meningitis are unknown. TNF-α and IL-1β were upregulated (real time PCR and in situ hybridization) in neurons and astrocytes time-dependently and maximally in the hippocampus during murine pneumococcal meningitis. Upregulation of TNF-α and IL-1β mRNA in the brain parenchyma was independent of cerebrospinal fluid leukocytosis, pneumococcal pneumolysin and H2O2, but it was potently induced by pneumococcal cell wall (PCW) fragments. Brain TNF-α mRNA was downregulated by a matrix metalloproteinases inhibitor. PCW fragments were located in the brain parenchyma. In conclusion, PCW fragments and matrix metalloproteinases trigger cytokine induction in the brain parenchyma during pneumococcal meningitis.",

keywords = "Brain, IL-1β, Meningitis, Pneumococcal cell wall, S. pneumoniae, TNF-α",

author = "Kaywan Izadpanah and Dorette Freyer and Weber, {Joerg R.} and Braun, {Johann S.}",

note = "Funding Information: This work was supported by the Deutsche Forschungsgemeinschaft ( SFB 507 ) and the Transregio ( SFB 43 ). Publisher Copyright: {\textcopyright} 2014 Elsevier B.V.",

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doi = "10.1016/j.jneuroim.2014.08.625",

language = "English",

volume = "276",

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journal = "Journal of Neuroimmunology",

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T1 - Brain parenchymal TNF-α and IL-1β induction in experimental pneumococcal meningitis

AU - Izadpanah, Kaywan

AU - Freyer, Dorette

AU - Weber, Joerg R.

AU - Braun, Johann S.

PY - 2014/11/15

Y1 - 2014/11/15

N2 - Triggers of brain inflammation in pneumococcal meningitis are unknown. TNF-α and IL-1β were upregulated (real time PCR and in situ hybridization) in neurons and astrocytes time-dependently and maximally in the hippocampus during murine pneumococcal meningitis. Upregulation of TNF-α and IL-1β mRNA in the brain parenchyma was independent of cerebrospinal fluid leukocytosis, pneumococcal pneumolysin and H2O2, but it was potently induced by pneumococcal cell wall (PCW) fragments. Brain TNF-α mRNA was downregulated by a matrix metalloproteinases inhibitor. PCW fragments were located in the brain parenchyma. In conclusion, PCW fragments and matrix metalloproteinases trigger cytokine induction in the brain parenchyma during pneumococcal meningitis.

AB - Triggers of brain inflammation in pneumococcal meningitis are unknown. TNF-α and IL-1β were upregulated (real time PCR and in situ hybridization) in neurons and astrocytes time-dependently and maximally in the hippocampus during murine pneumococcal meningitis. Upregulation of TNF-α and IL-1β mRNA in the brain parenchyma was independent of cerebrospinal fluid leukocytosis, pneumococcal pneumolysin and H2O2, but it was potently induced by pneumococcal cell wall (PCW) fragments. Brain TNF-α mRNA was downregulated by a matrix metalloproteinases inhibitor. PCW fragments were located in the brain parenchyma. In conclusion, PCW fragments and matrix metalloproteinases trigger cytokine induction in the brain parenchyma during pneumococcal meningitis.

KW - Brain

KW - IL-1β

KW - Meningitis

KW - Pneumococcal cell wall

KW - S. pneumoniae

KW - TNF-α

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Brain parenchymal TNF-α and IL-1β induction in experimental pneumococcal meningitis

Abstract

Keywords

ASJC Scopus subject areas

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