TY - JOUR
T1 - Calcitonin gene-related peptides relax guinea pig and rat gastric smooth muscle
AU - Katsoulis, Stavros
AU - Conlon, J. Michael
N1 - Funding Information:
The work was supported by the Stiftung Volkswagenwerk.
PY - 1989/3/14
Y1 - 1989/3/14
N2 - Rat and human α- and β-calcitonin gene-related peptide (CGRP), in the concentration range 1-100 nM, produced sustained relaxations of longitudinal muscle from the rat fundus and guinea pig gastric corpus. The peptides were equipotent and equally effective. Tetrodotoxin, adrenoceptor and purine receptor antagonists, somatostatin, apamin and Tyr-rat α-CGRP-(28-37) peptide did not modify the action of the CGRP peptides. The CGRP-induced responses were inhibited by verapamil and potentiated by Bay K-8644. Incubation of the tissues with indomethacin markedly reduced the magnitude of the CGRP- and adrenaline-induced relaxations, but their responsiveness was restored by addition of prostaglandins E1, E2 and E2α in concentrations that alone did not affect the motility of the indomethacin-treated strips. It is suggested that an inhibitory receptor for CGRP on gastric smooth muscle cells is linked to calcium channels and may be activated or sensitized by endogenous prostaglandins.
AB - Rat and human α- and β-calcitonin gene-related peptide (CGRP), in the concentration range 1-100 nM, produced sustained relaxations of longitudinal muscle from the rat fundus and guinea pig gastric corpus. The peptides were equipotent and equally effective. Tetrodotoxin, adrenoceptor and purine receptor antagonists, somatostatin, apamin and Tyr-rat α-CGRP-(28-37) peptide did not modify the action of the CGRP peptides. The CGRP-induced responses were inhibited by verapamil and potentiated by Bay K-8644. Incubation of the tissues with indomethacin markedly reduced the magnitude of the CGRP- and adrenaline-induced relaxations, but their responsiveness was restored by addition of prostaglandins E1, E2 and E2α in concentrations that alone did not affect the motility of the indomethacin-treated strips. It is suggested that an inhibitory receptor for CGRP on gastric smooth muscle cells is linked to calcium channels and may be activated or sensitized by endogenous prostaglandins.
KW - (Rat, Guinea pig)
KW - Ca channels
KW - Calcitonin gene-related peptide (CGRP)
KW - Prostaglandins
KW - Stomach
UR - http://www.scopus.com/inward/record.url?scp=0024555698&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024555698&partnerID=8YFLogxK
U2 - 10.1016/0014-2999(89)90612-2
DO - 10.1016/0014-2999(89)90612-2
M3 - Article
C2 - 2470600
AN - SCOPUS:0024555698
SN - 0014-2999
VL - 162
SP - 129
EP - 134
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 1
ER -