TY - JOUR
T1 - CAM 17.1 - A new diagnostic marker in pancreatic cancer
AU - Gansauge, F.
AU - Gansauge, S.
AU - Parker, N.
AU - Beger, M. I.
AU - Poch, B.
AU - Link, K. H.
AU - Safi, F.
AU - Beger, Hans G.
PY - 1996
Y1 - 1996
N2 - CAM 17.1-Ab is a recently described monoclonal antibody that detects a mucus glycoprotein with high specificity for intestinal mucus, particularly in the colon, small intestine, biliary tract and pancreas. We investigated the expression and release of CAM 17.1 in pancreatic carcinoma cell lines and tissue specimens of normal pancreas, chronic pancreatitis and pancreatic cancer. CAM 17.1 was weakly expressed on normal ductal cells and chronic pancreatitis, whereas it was overexpressed in pancreatic cancer. Serum analysis using a new enzyme-linked antibody sandwich assay (CAM 17.1/WGA) of patients with chronic pancreatitis, pancreatic cancer or other gastrointestinal cancer and of healthy blood donors revealed a high sensitivity (67%) and excellent specificity (90%) of CAM 17.1/WGA assay in pancreatic cancer. In comparison with the tumour marker CA19-9, the sensitivity of the CAM 17.1/WGA assay was similar to the sensitivity of CA 19-9 (67% and 76%, P = 0.22), whereas the specificity of CAM 17.1/WGA assay was higher than in CA 19-9 (90% compared with 78% in chronic pancreatitis, P>0.05).
AB - CAM 17.1-Ab is a recently described monoclonal antibody that detects a mucus glycoprotein with high specificity for intestinal mucus, particularly in the colon, small intestine, biliary tract and pancreas. We investigated the expression and release of CAM 17.1 in pancreatic carcinoma cell lines and tissue specimens of normal pancreas, chronic pancreatitis and pancreatic cancer. CAM 17.1 was weakly expressed on normal ductal cells and chronic pancreatitis, whereas it was overexpressed in pancreatic cancer. Serum analysis using a new enzyme-linked antibody sandwich assay (CAM 17.1/WGA) of patients with chronic pancreatitis, pancreatic cancer or other gastrointestinal cancer and of healthy blood donors revealed a high sensitivity (67%) and excellent specificity (90%) of CAM 17.1/WGA assay in pancreatic cancer. In comparison with the tumour marker CA19-9, the sensitivity of the CAM 17.1/WGA assay was similar to the sensitivity of CA 19-9 (67% and 76%, P = 0.22), whereas the specificity of CAM 17.1/WGA assay was higher than in CA 19-9 (90% compared with 78% in chronic pancreatitis, P>0.05).
KW - Cell lines
KW - Pancreas
KW - Tumour marker
UR - http://www.scopus.com/inward/record.url?scp=0030457608&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030457608&partnerID=8YFLogxK
U2 - 10.1038/bjc.1996.666
DO - 10.1038/bjc.1996.666
M3 - Article
C2 - 8980403
AN - SCOPUS:0030457608
SN - 0007-0920
VL - 74
SP - 1997
EP - 2002
JO - British Journal of Cancer
JF - British Journal of Cancer
IS - 12
ER -