Carboxyfluorescein and biotin neuromedin C analogues: Synthesis and applications

Brian Walker, Joseph Gray, Deirdre M. Burns, Oiming Wang, Thomas E. Adrian, David H. Nichols, Richard F. Murphy, John Nelson

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Two neuromedin C (NC) analogues were constructed by Fmoc synthesis and in situ coupling of 4(5)-carboxyfluorescein or biotin to the N-terminus. Both displayed full agonism in an amylase release assay and cross-reacted fully with a NC-specific antiserum. Biotin NC functioned in a strepavidin-capture ELISA. Carboxyfluorescein NC was used to probe receptor localization in rat stomach. Specific NC binding sites, which did not interact with substance P, angiotensin I, or neurokinin A, were labeled in the antrum. Identity of NC binding sites was confirmed by microautoradiography. The specifically labeled cells were all found in the lamina propria and at least some of cells were identified as eosinophils.

Original languageEnglish
Pages (from-to)255-261
Number of pages7
JournalPeptides
Volume16
Issue number2
DOIs
Publication statusPublished - 1995
Externally publishedYes

Keywords

  • 4(5)-Carboxyfluorescein neuromedin C
  • Biotin neuromedin C
  • Cos-7 cells
  • Eosinophils
  • Lamina propria
  • Neuromedin C receptor localization
  • Peptide synthesis
  • Rat stomach
  • Streptavidin-capture immunoassay

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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