Cellular bioenergetics, caspase activity and glutathione in murine lungs infected with influenza A virus

Ahmed R. Alsuwaidi, Saeeda Almarzooqi, Alia Albawardi, Sheela Benedict, Jose Kochiyil, Farah Mustafa, Stacey M. Hartwig, Steven M. Varga, Abdul Kader Souid

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Inhibition of cellular respiration, oxidation of glutathione and induction of apoptosis have been reported in epithelial cells infected in vitro with influenza A virus (IAV). Here, the same biomarkers were investigated in vivo by assessing the lungs of BALB/c mice infected with IAV. Cellular respiration declined on day 3 and recovered on day 7 post-infection. For days 3-5, the rate (mean±SD) of respiration (μMO2min-1mg-1) in uninfected lungs was 0.103±0.021 (n=4) and in infected lungs was 0.076±0.025 (n=4, p=0.026). Relative cellular ATP (infected/uninfected) was 4.7 on day 2 and 1.07 on day 7. Intracellular caspase activity peaked on day 7. Cellular glutathione decreased by ≥10% on days 3-7. Lung pathology was prominent on day 3 and caspase-3 labeling was prominent on day 5. IAV infection was associated with suppression of cellular respiration, diminished glutathione, and induction of apoptosis. These functional biomarkers were associated with structural changes noted in infected mice.

Original languageEnglish
Pages (from-to)180-188
Number of pages9
JournalVirology
Volume446
Issue number1-2
DOIs
Publication statusPublished - Nov 2013

Keywords

  • Caspase-3
  • Cellular ATP
  • Cellular bioenergetics
  • Cellular glutathione
  • Cellular respiration
  • Influenza A virus

ASJC Scopus subject areas

  • Virology

Fingerprint

Dive into the research topics of 'Cellular bioenergetics, caspase activity and glutathione in murine lungs infected with influenza A virus'. Together they form a unique fingerprint.

Cite this