Cellulases and hemicellulases of the anaerobic fungus Piromyces constitute a multiprotein cellulose-binding complex and are encoded by multigene families

Bassam R.S. Ali, Liqing Zhou, Fiona M. Graves, Robert B. Freedman, Gary W. Black, Harry J. Gilbert, Geoffrey P. Hazlewood

Research output: Contribution to journalArticlepeer-review

75 Citations (Scopus)

Abstract

More than 80% of the extracellular Avicelase, endoglucanase, xylanase and mannanase activities of the anaerobic fungus Piromyces were associated with a cellulose-binding complex. The complex was composed of at least 10 polypeptides ranging in size from 190 kDa to 50 kDa, and contained numerous endoglucanases, xylanases and mannanases. Multiple genes encoding each of these activities were isolated from an expressing cDNA library.

Original languageEnglish
Pages (from-to)15-21
Number of pages7
JournalFEMS Microbiology Letters
Volume125
Issue number1
DOIs
Publication statusPublished - Jan 1 1995
Externally publishedYes

Keywords

  • Anaerobic fungus
  • Cellulase
  • Cellulose-binding complex
  • Hemicellulase
  • Piromyces sp
  • Polysaccharide Hydrolase genes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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