Changes of pH affect calcium currents but not outward potassium currents in rat myometrial cells

A. V. Shmigol, R. D. Smith, M. J. Taggart, Susan Wray, D. A. Eisner

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Spontaneous contraction of uterine smooth muscle is enhanced by alkalinization and depressed by acidification. We have investigated the ionic currents responsible for this in single myometrial cells. Intracellular acidification (20 mM butyrate) at constant external pH depressed the magnitude of the calcium current to 58±6% of control, but had little effect on outward currents. Similar but slower effects were also observed when the extracellular pH was lowered to 6.9 (56±9% of control). Correspondingly, when the intracellular or extracelluar pH was elevated (20 mM NH4Cl or pH 7.9 respectively) the calcium current magnitude increased (165±15 % in NH4Cl; 136±2 % at pH 7.9) and there was, again, no effect on the outward currents. These observations are consistent with the effects of pH on spontaneous contractile activity being due to an effect on the membrane calcium current.

Original languageEnglish
Pages (from-to)135-137
Number of pages3
JournalPflügers Archiv European Journal of Physiology
Volume431
Issue number1
DOIs
Publication statusPublished - Nov 1995
Externally publishedYes

Keywords

  • calcium current
  • pH
  • potassium current
  • uterus

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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