Characterization and bioactive properties of exopolysaccharides produced by Streptococcus thermophilus and Lactobacillus bulgaricus isolated from labaneh

Anas A. Al-Nabulsi; Ziad W. Jaradat; Farah R. Al Qudsi; Lina Elsalem; Tareq M. Osaili; Amin N. Olaimat; Gennaro Esposito; Shao Quan Liu; Mutamed M. Ayyash

doi:10.1016/j.lwt.2022.113817

Characterization and bioactive properties of exopolysaccharides produced by Streptococcus thermophilus and Lactobacillus bulgaricus isolated from labaneh

Anas A. Al-Nabulsi, Ziad W. Jaradat, Farah R. Al Qudsi, Lina Elsalem, Tareq M. Osaili, Amin N. Olaimat, Gennaro Esposito, Shao Quan Liu, Mutamed M. Ayyash

Department of Food, Nutrition and Health

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

This study aimed to investigate and characterize the bioactivities of two novel exopolysaccharides, EPS-S and EPS-L, produced respectively by Streptococcus thermophilus and Lactobacillus bulgaricus that were newly isolated from labaneh. Both EPSs are composed of glucose and other sugars, with average molecular weights of 537.80 kDa and 248.15 kDa, ZP of −26.7 and −44.83 mV, and particle sizes of 824.5 and 757.9 nm IC₅₀ values of EPS-S and EPS-L against colon cancer cells (HT-29, HCT-116) were 5.01 and 0.79 mg/mL and 4.20 and 3.46 mg/mL, respectively. Both EPSs displayed a bactericidal effect against Listeria monocytogenes, while EPS-L exhibited significant inhibition of methicillin-resistant Staphylococcus aureus. EPS-S inhibited 53.8% of α-amylase, 9.2% of α-glucosidase, and 25.9% of angiotensin-converting enzyme (ACE), while EPS-L inhibited 47.8% of α-amylase, 11.9% of α-glucosidase, and 52.5% of ACE. This study reveals promising biological properties of EPS-S and EPS-L for potential applications in the food and nutraceutical industries.

Original language	English
Article number	113817
Journal	LWT
Volume	167
DOIs	https://doi.org/10.1016/j.lwt.2022.113817
Publication status	Published - Sept 15 2022

Keywords

ACE-Inhibition
Anticancer
Antidiabetic
Antioxidants
Exopolysaccharide

ASJC Scopus subject areas

Food Science

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10.1016/j.lwt.2022.113817

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@article{26a81c2f0aec465f96fe5a9141efbd03,

title = "Characterization and bioactive properties of exopolysaccharides produced by Streptococcus thermophilus and Lactobacillus bulgaricus isolated from labaneh",

abstract = "This study aimed to investigate and characterize the bioactivities of two novel exopolysaccharides, EPS-S and EPS-L, produced respectively by Streptococcus thermophilus and Lactobacillus bulgaricus that were newly isolated from labaneh. Both EPSs are composed of glucose and other sugars, with average molecular weights of 537.80 kDa and 248.15 kDa, ZP of −26.7 and −44.83 mV, and particle sizes of 824.5 and 757.9 nm IC50 values of EPS-S and EPS-L against colon cancer cells (HT-29, HCT-116) were 5.01 and 0.79 mg/mL and 4.20 and 3.46 mg/mL, respectively. Both EPSs displayed a bactericidal effect against Listeria monocytogenes, while EPS-L exhibited significant inhibition of methicillin-resistant Staphylococcus aureus. EPS-S inhibited 53.8% of α-amylase, 9.2% of α-glucosidase, and 25.9% of angiotensin-converting enzyme (ACE), while EPS-L inhibited 47.8% of α-amylase, 11.9% of α-glucosidase, and 52.5% of ACE. This study reveals promising biological properties of EPS-S and EPS-L for potential applications in the food and nutraceutical industries.",

keywords = "ACE-Inhibition, Anticancer, Antidiabetic, Antioxidants, Exopolysaccharide",

author = "Al-Nabulsi, {Anas A.} and Jaradat, {Ziad W.} and {Al Qudsi}, {Farah R.} and Lina Elsalem and Osaili, {Tareq M.} and Olaimat, {Amin N.} and Gennaro Esposito and Liu, {Shao Quan} and Ayyash, {Mutamed M.}",

note = "Funding Information: Authors are thankful to Jordan University of Science and Technology and United Arab Emirates University for funding this project. We thank NYUAD CTP for the access to the NMR facility. Publisher Copyright: {\textcopyright} 2022 The Author(s)",

year = "2022",

month = sep,

day = "15",

doi = "10.1016/j.lwt.2022.113817",

language = "English",

volume = "167",

journal = "LWT - Food Science and Technology",

issn = "0023-6438",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Characterization and bioactive properties of exopolysaccharides produced by Streptococcus thermophilus and Lactobacillus bulgaricus isolated from labaneh

AU - Al-Nabulsi, Anas A.

AU - Jaradat, Ziad W.

AU - Al Qudsi, Farah R.

AU - Elsalem, Lina

AU - Osaili, Tareq M.

AU - Olaimat, Amin N.

AU - Esposito, Gennaro

AU - Liu, Shao Quan

AU - Ayyash, Mutamed M.

N1 - Funding Information: Authors are thankful to Jordan University of Science and Technology and United Arab Emirates University for funding this project. We thank NYUAD CTP for the access to the NMR facility. Publisher Copyright: © 2022 The Author(s)

PY - 2022/9/15

Y1 - 2022/9/15

N2 - This study aimed to investigate and characterize the bioactivities of two novel exopolysaccharides, EPS-S and EPS-L, produced respectively by Streptococcus thermophilus and Lactobacillus bulgaricus that were newly isolated from labaneh. Both EPSs are composed of glucose and other sugars, with average molecular weights of 537.80 kDa and 248.15 kDa, ZP of −26.7 and −44.83 mV, and particle sizes of 824.5 and 757.9 nm IC50 values of EPS-S and EPS-L against colon cancer cells (HT-29, HCT-116) were 5.01 and 0.79 mg/mL and 4.20 and 3.46 mg/mL, respectively. Both EPSs displayed a bactericidal effect against Listeria monocytogenes, while EPS-L exhibited significant inhibition of methicillin-resistant Staphylococcus aureus. EPS-S inhibited 53.8% of α-amylase, 9.2% of α-glucosidase, and 25.9% of angiotensin-converting enzyme (ACE), while EPS-L inhibited 47.8% of α-amylase, 11.9% of α-glucosidase, and 52.5% of ACE. This study reveals promising biological properties of EPS-S and EPS-L for potential applications in the food and nutraceutical industries.

AB - This study aimed to investigate and characterize the bioactivities of two novel exopolysaccharides, EPS-S and EPS-L, produced respectively by Streptococcus thermophilus and Lactobacillus bulgaricus that were newly isolated from labaneh. Both EPSs are composed of glucose and other sugars, with average molecular weights of 537.80 kDa and 248.15 kDa, ZP of −26.7 and −44.83 mV, and particle sizes of 824.5 and 757.9 nm IC50 values of EPS-S and EPS-L against colon cancer cells (HT-29, HCT-116) were 5.01 and 0.79 mg/mL and 4.20 and 3.46 mg/mL, respectively. Both EPSs displayed a bactericidal effect against Listeria monocytogenes, while EPS-L exhibited significant inhibition of methicillin-resistant Staphylococcus aureus. EPS-S inhibited 53.8% of α-amylase, 9.2% of α-glucosidase, and 25.9% of angiotensin-converting enzyme (ACE), while EPS-L inhibited 47.8% of α-amylase, 11.9% of α-glucosidase, and 52.5% of ACE. This study reveals promising biological properties of EPS-S and EPS-L for potential applications in the food and nutraceutical industries.

KW - ACE-Inhibition

KW - Anticancer

KW - Antidiabetic

KW - Antioxidants

KW - Exopolysaccharide

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U2 - 10.1016/j.lwt.2022.113817

DO - 10.1016/j.lwt.2022.113817

M3 - Article

AN - SCOPUS:85135722311

SN - 0023-6438

VL - 167

JO - LWT - Food Science and Technology

JF - LWT - Food Science and Technology

M1 - 113817

ER -

Characterization and bioactive properties of exopolysaccharides produced by Streptococcus thermophilus and Lactobacillus bulgaricus isolated from labaneh

Abstract

Keywords

ASJC Scopus subject areas

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