TY - JOUR
T1 - Characterization of high-affinity protein D binding sites on the surface of rat epididymal spermatozoa
AU - Hall, Joseph C.
AU - Tubbs, Christopher E.
AU - Li, Yuehua
AU - Ashraf, Salman
PY - 1996
Y1 - 1996
N2 - 125I-labeled protein D was shown to bind to whole rat epididymal sperm in a time-dependent, saturable, and reversible manner. Scatchard analysis indicates that there are 3.4 x 105 binding sites/sperm cell with a binding efficiency (K(d)) of 2.3 x 10-10 M. Binding of protein D to sperm reaches equilibrium by 95 min. and is reversible by 30 min. Specific binding of protein D to sperm pre-treated with a dilute trypsin solution is reduced significantly when compared with controls. Our findings establish that protein D-specific binding proteins displaying characteristics of a true receptor are present on the surface of rat epididymal sperm.
AB - 125I-labeled protein D was shown to bind to whole rat epididymal sperm in a time-dependent, saturable, and reversible manner. Scatchard analysis indicates that there are 3.4 x 105 binding sites/sperm cell with a binding efficiency (K(d)) of 2.3 x 10-10 M. Binding of protein D to sperm reaches equilibrium by 95 min. and is reversible by 30 min. Specific binding of protein D to sperm pre-treated with a dilute trypsin solution is reduced significantly when compared with controls. Our findings establish that protein D-specific binding proteins displaying characteristics of a true receptor are present on the surface of rat epididymal sperm.
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U2 - 10.1080/15216549600201633
DO - 10.1080/15216549600201633
M3 - Article
C2 - 8955890
AN - SCOPUS:0029906927
SN - 1039-9712
VL - 40
SP - 1003
EP - 1010
JO - Biochemistry and Molecular Biology International
JF - Biochemistry and Molecular Biology International
IS - 5
ER -