TY - JOUR
T1 - Characterization of melanotropin-release-inhibiting factor (melanostatin) from frog brain
T2 - Homology with human neuropeptide Y
AU - Chartrel, N.
AU - Conlon, J. M.
AU - Danger, J. M.
AU - Fournier, A.
AU - Tonon, M. C.
AU - Vaudry, H.
PY - 1991
Y1 - 1991
N2 - A polypeptide was purified from frog brain extracts on the basis of its ability to inhibit α-melanotropin release from perifused frog neurointermediate lobes. Based on Edman degradation, amino acid analysis, and peptide mapping, the primary structure of this frog melanotropin-release-inhibiting factor (melanostatin) was determined to be H-Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Ala-Glu-Asp -Met-Ala-Lys-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg- Gln-Arg-Tyr-NH2. Frog melanostatin belongs to the pancreatic polypeptide/neuropeptide Y/peptide YY family, and the structure of this peptide differs from that of human neuropeptide Y by only one amino acid substitution in position 19. A synthetic replicate of frog melanostatin is coeluted with the native peptide on HPLC and is highly potent in inhibiting α-melanotropin secretion in vitro (IC50 = 60 nM).
AB - A polypeptide was purified from frog brain extracts on the basis of its ability to inhibit α-melanotropin release from perifused frog neurointermediate lobes. Based on Edman degradation, amino acid analysis, and peptide mapping, the primary structure of this frog melanotropin-release-inhibiting factor (melanostatin) was determined to be H-Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Ala-Glu-Asp -Met-Ala-Lys-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg- Gln-Arg-Tyr-NH2. Frog melanostatin belongs to the pancreatic polypeptide/neuropeptide Y/peptide YY family, and the structure of this peptide differs from that of human neuropeptide Y by only one amino acid substitution in position 19. A synthetic replicate of frog melanostatin is coeluted with the native peptide on HPLC and is highly potent in inhibiting α-melanotropin secretion in vitro (IC50 = 60 nM).
KW - control of pigmentation
KW - evolution
KW - peptide isolation
KW - peptide mapping
KW - pituitary melanotrophs
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U2 - 10.1073/pnas.88.9.3862
DO - 10.1073/pnas.88.9.3862
M3 - Article
C2 - 1673794
AN - SCOPUS:0025760743
SN - 0027-8424
VL - 88
SP - 3862
EP - 3866
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 9
ER -