TY - JOUR
T1 - Characterization of novel calcium compounds from tilapia (Oreochromis niloticus) by-products and their effects on proliferation and differentiation of MC3T3-E1 cells
AU - Aenglong, Chakkapat
AU - Ngasakul, Nujamee
AU - Limpawattana, Maruj
AU - Sukketsiri, Wanida
AU - Chockchaisawasdee, Suwimol
AU - Stathopoulos, Costas
AU - Tanasawet, Supita
AU - Klaypradit, Wanwimol
N1 - Funding Information:
This work was financially supported by the Office of the Ministry of Higher Education, Science, Research and Innovation; and the Thailand Science Research and Innovation through the Kasetsart University Reinventing University Program 2021 and European Union's Horizon 2020 Research and Innovation Program [grant number 952594, ERA Chair project DRIFT-FOOD].
Funding Information:
This work was financially supported by the Office of the Ministry of Higher Education, Science, Research and Innovation; and the Thailand Science Research and Innovation through the Kasetsart University Reinventing University Program 2021 and European Union’s Horizon 2020 Research and Innovation Program [grant number 952594, ERA Chair project DRIFT-FOOD].
Publisher Copyright:
© 2022
PY - 2023/1
Y1 - 2023/1
N2 - This study aimed to produce and characterize of a powder mixture from tilapia (Oreochromis niloticus) bone resulting from the addition to hydroxyapatite (HA) of various acids (lactic acid, citric acid, and malic acid). Structured hydroxyapatite lactate compound (SHA-Lac) and SHA-citrate malate compound (SHA-CiMa) were water-soluble and Ca and P were the most abundant minerals contained in those compounds had main elements in Ca and P. FT-IR indicated that COOH played an important role for Ca2+ binding. XRD patterns indicated these compounds were amorphous crystal. For bioavailability on preosteoblast MC3T3-E1 cells, all samples at 100 μg/mL showed no cytotoxicity, no change in morphology, and no cell apoptosis, with the percentage of cell viability higher than 80%. For ability of mineral deposition at 7, 14, and 21 days of culture, all samples significantly increased the mineralization of MC3T3-E1osteoblast cells, thereby resulting in rising of the proliferation and differentiation during the early stages.
AB - This study aimed to produce and characterize of a powder mixture from tilapia (Oreochromis niloticus) bone resulting from the addition to hydroxyapatite (HA) of various acids (lactic acid, citric acid, and malic acid). Structured hydroxyapatite lactate compound (SHA-Lac) and SHA-citrate malate compound (SHA-CiMa) were water-soluble and Ca and P were the most abundant minerals contained in those compounds had main elements in Ca and P. FT-IR indicated that COOH played an important role for Ca2+ binding. XRD patterns indicated these compounds were amorphous crystal. For bioavailability on preosteoblast MC3T3-E1 cells, all samples at 100 μg/mL showed no cytotoxicity, no change in morphology, and no cell apoptosis, with the percentage of cell viability higher than 80%. For ability of mineral deposition at 7, 14, and 21 days of culture, all samples significantly increased the mineralization of MC3T3-E1osteoblast cells, thereby resulting in rising of the proliferation and differentiation during the early stages.
KW - MC3T3-E1 cells
KW - Soluble calcium
KW - Structured hydroxyapatite
KW - Tilapia bones
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U2 - 10.1016/j.jff.2022.105361
DO - 10.1016/j.jff.2022.105361
M3 - Article
AN - SCOPUS:85144074460
SN - 1756-4646
VL - 100
JO - Journal of Functional Foods
JF - Journal of Functional Foods
M1 - 105361
ER -